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Genetic characterization and linkage analysis of spotted leaf 6, liguleless and lax panicle traits in mutant rice

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Abstract

Phenotypic mutants are valuable resources for elucidating the function of genes responsible for their expression. This study examined mutant rice strains expressing three traits: spotted leaf 6 (spl6), lax panicle (lax), and liguleless (lg). In the mutant, the spl6 phenotype was a genetically programmed lesion-mimicking mutation (LMM) that displayed spontaneously scattered spots across the leaf surface. In the lg trait, the plant lacked a collar region, and there were no auricles and ligules at the junction of the leaf blade and leaf sheath. The lax panicle trait manifested as sparely arranged spikelets resulting from the terminal spikelet with no lateral spikelets, which caused a drastic reduction of the total seed number in the mutant. All three mutant genes were genetically recessive and had nuclear gene regulation. The dihybrid segregation of the lg gene was classified independently according to the Mendelian 9:3:3:1 dihybrid segregation ratio in the F2 generation, suggesting that the lg gene is not linked to the same chromosome as the lax and spl6 genes. On the other hand, spl6 and lax were not assorted independently, indicating that they are closely linked on chromosome 1 in rice. Additional linkage analysis from the recombination of spl6 and lax genes reconfirmed that the two genes were ~9.4 cM away from each other. The individual single-gene mutant plant from one plant with a three-gene mutation (spl6, lax, and lg) was isolated and characterized, which will be a crucial resource for the gene cloning and molecular characterization of these genes.

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Modified from Yoshimura et al. (1997).

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Authors and Affiliations

  1. Department of Biotechnology, Institute of Biotechnology, College of Life and Applied Sciences, Yeungnam University, Gyeongsan, 38541, Republic of Korea

    Mohammad Nurul Matin & Sang Gu Kang

  2. Molecular Genetics Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Mohammad Nurul Matin

  3. Sunforce Inc, 208-31, Gumchang-ro, Yeungcheon-si, Gyeongsangbuk-do, 31882, Republic of Korea

    Kyung Eun Lee

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MNM: conceptualization, methodology, data curation, formal analysis, original draft preparation and writing. KEL: methodology and editing. SGK: conceptualization, investigation, resources, visualization, supervision, writing and editing. All authors have read and agreed for the publication of the article.

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Correspondence to Sang Gu Kang.

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Ad-hoc editor: Hitendra Kumar Patel

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Matin, M.N., Lee, K.E. & Kang, S.G. Genetic characterization and linkage analysis of spotted leaf 6, liguleless and lax panicle traits in mutant rice. J Genet 103, 16 (2024). https://doi.org/10.1007/s12041-024-01466-w

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