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Characterization and fine mapping of a lesion mimic mutant (Lm5) with enhanced stripe rust and powdery mildew resistance in bread wheat (Triticum aestivum L.)

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A novel light intensity-dependent lesion mimic mutant with enhanced disease resistance was physiologically, biochemically, and genetically characterized, and the causative gene was fine mapped to a 1.28 Mbp interval containing 17 high-confidence genes.

Abstract

Lesion mimic mutants are ideal for studying disease resistance and programmed cell death photosynthesis in plants to improve crop yield. In this study, a novel light intensity-dependent lesion mimic mutant (MC21) was obtained from the wheat variety Chuannong16 (CN16) by ethyl methane sulfonate treatment. The mutant initially developed tiny lesion spots on the basal part of the leaves, which then gradually proceeded down to leaf sheaths, stems, shells, and awns at the flowering stage. The major agronomic traits were significantly altered in the mutant compared to that in the wild-type CN16. Furthermore, the mutant exhibited a lesion phenotype with degenerated chloroplast structure, decreased chlorophyll content, increased level of reactive oxygen species, and increased resistance to stripe rust and powdery mildew. Genetic analysis indicated that the lesion phenotype was controlled by a novel single semi-dominant nuclear gene. The target gene was mapped on chromosome arm 2AL located between Kompetitive Allele Specific PCR (KASP) markers, KASP-4211 and KASP-5353, and tentatively termed as lesion mimic 5 (Lm5). The fine mapping suggested that Lm5 was located in a 1.28 Mbp interval between markers KASP-5825 and KASP-9366; 17 high-confidence candidate genes were included in this genomic region. This study provides an important foundational step for further cloning of Lm5 using a map-based approach.

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Acknowledgements

This work is supported by the National Natural Science Foundation of China (31970243 and 31971937), the Basic Research Project of Science and Technology Plan of Guizhou Province (ZK [2021] general 131), National Undergraduate Innovation Training Program (202010626001), the International Science and Technology Cooperation and Exchanges Program of Science and Technology Department of Sichuan Province (2021YFH0083), and the Applied Basic Research Programs of Science and Technology Department of Sichuan Province (2020YJ0140 and 2021YJ0503), We thank the anonymous referees for reading and revising this manuscript.

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  1. Cong Li and Hang Liu have contributed equally to this work.

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  1. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China

    Cong Li, Hang Liu, Jian Wang, Yang Mu, Huaping Tang, Qiang Xu, Qiantao Jiang, Yaxi Liu, Pengfei Qi, Lin Huang, Guoyue Chen, Jirui Wang, Yuming Wei, Youliang Zheng, Lulu Gou, Xiujin Lan & Jian Ma

  2. College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China

    Qi Pan, Yue Wang, Kunyan Wu & Peiying Jia

  3. College of Agronomy, Shanxi Agricultural University, Taiyuan, 030031, China

    Xiaojun Zhang

  4. College of Agroforestry Engineering and Planning/Guizhou Key Laboratory of Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren, 554300, China

    Qifu Yao

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  1. Cong Li
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Contributions

CL and HL performed the entire study and drafted this manuscript. JW, QP, KYW, YW, and PYJ did phenotype measurement and data analysis. YM and HPT did field work and data analysis. QX, QTJ, YXL, PFQ, XJZ, LH collected and analyzed data. GYC, JRW, and YMW, YLZ did QTL analysis and manuscript revision. LLG and QFY revised the manuscript. XJL guided the study and revised the manuscript. JM designed the experiments, guided the entire study, participated in data analysis, wrote and extensively revised this manuscript. All authors participated in the research and approved the final manuscript.

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Correspondence to Qifu Yao, Xiujin Lan or Jian Ma.

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All experiments and data analyses were conducted in Sichuan. All authors contributed to the study and approved the final version for submission. The manuscript has not been submitted to any other journal.

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Communicated by Reem Aboukhaddour.

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Li, C., Liu, H., Wang, J. et al. Characterization and fine mapping of a lesion mimic mutant (Lm5) with enhanced stripe rust and powdery mildew resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet 135, 421–438 (2022). https://doi.org/10.1007/s00122-021-03973-1

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