Summary
To obtain genetic materials to breed qualitatively improved rice storage proteins, we screened about 3,000 mutant lines induced by the treatment of rice fertilized egg cell with N-methyl-N-nitrosourea (MNU). The screening was performed by comparing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with that of the original variety, Kinmaze, especially focussing on the changes in polypeptides present in two kinds of protein bodies, PB-I and PB-II. We selected 17 mutant lines and classified them into 4 types on the basis of variations of the relative contents of the polypeptides. Determination of extracted protein in the starchy endosperm of the mutants revealed changes in the content of prolamin and glutelin but not globulin. In some mutants there was marked accumulation of 57 kDa polypeptide concomitant with the remarkable reduction of glutelin subunits. Treatment of the fertilized egg cell with MNU was found to be an effective method to induce mutations for storage proteins in protein bodies of rice.
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References
Beachell HM, Khush OS, Juliano BO (1972) Breeding for high protein content in rice. In: Rice Breeding. Int Rice Res Inst, Los Baños, Philippines, pp 419–428
Cagampang GB, Cruz LJ, Espiritu SG, Santiago RG, Juliano BO (1966) Studies on the extraction and composition of rice proteins. Cereal Chem 43:145–155
Higashi T, Kushibuchi K, Ito R (1974) Studies on breeding for high protein rice. I. Protein content of different rice varieties and their relations with some agronomic traits including yield. Jpn J Breed 24:88–96
Juliano BO (1972) The rice caryopsis and its composition. In: Houston DF (ed) Rice chemistry and technology. Am Assoc Cereal Chem, St. Paul MN, pp 16–74
Kambayashi M, Tsurumi I, Sasahara T (1984) Genetic studies on improvement of protein content in rice grain. Jpn J Breed 34:356–363
Kitamura K, Kaizuma N (1981) Mutant strains with low level of subunits of 7S globulin in soybean (Glycine max Merr.) seed. Jpn J Breed 31:353–359
Krishnan HB, Franceschi VR, Okita TW (1986) Immunochemical studies on the role of the Golgi complex in proteinbody formation in rice seeds. Planta 169:471–480
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Matsuo T, Satoh H, Yoon KM, Omura T (1987) Oil content and fatty acid composition of a giant embryo mutant in rice. Jpn J Breed 37:185–191
Mertz ET, Bates LS, Nelson OE (1964) Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145:279–280
Munck L, Karlsson KE, Hagberg A, Eggum BO (1970) Gene for improved nutritional value in barley seed protein. Science 168:985–987
Nelson OE, Mertz ET, Bates LS (1965) Second mutant gene affecting the amino acid pattern of maize endosperm proteins. Science 150:1469–1470
O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
Ogawa M, Kumamaru T, Satoh H, Iwata N, Omura T, Kasai Z, Tanaka K (1987) Purification of protein body-I of rice seed and its polypeptide composition. Plant Cell Physiol 28:1517–1527
Omura T, Satoh H (1984) Mutation of grain properties in rice. In: Tsunoda S, Takahashi N (eds) Biology of rice. Jpn Sci Soc Press, Tokyo/Elsevier, Amsterdam, pp 293–303
Osborn TB (1924) The Vegetable Proteins, 2nd edn. Longmans, Green and Co, London
Osone K, Takagi T (1970) Studies on breeding for high protein content and quality in rice. I. Estimation of seed-protein content using the Dye-binding method. Jpn J Breed 20:301–304
Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 1. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236
Pernollet JC, Mossé J (1983) Structural and location of legume and cereal seed storage proteins. In: Daussant J, Mossé J, Vaughan J (eds) Seed protein. Academic Press, London New York, pp 155–191
Robert LS, Nozzolillo C, Altosaar I (1983) Molecular weight and charge heterogeneity of prolamins (Avenins) from nine oat (Avena sativa L.) cultivars of different protein content and from developing seeds. Cereal Chem 60:438–442
Sarker SC, Ogawa M, Takahashi M, Asada K (1986) Processing of a 57-kDa precursor peptide to subunits of rice glutelin. Plant Cell Physiol 27:1579–1586
Satoh H, Omura T (1979) Induction of mutation by the treatment of fertilized egg cell with N-methyl-N-nitrosourea in rice. J Fac Agric Kyushu Univ 24:165–174
Satoh H, Omura T (1981) New endosperm mutations induced by chemical mutagens in rice, Oryza sativa L. Jpn J Breed 31:316–326
Shewry PR, Ellis JRS, Pratt HM, Miflin BJ (1978) A comparison of methods for the extraction and separation of hordein fractions from 29 barley varieties. J Sci Food Agric 29:433–441
Shin YB, Tanaka S, Katayama T (1977) Studies on the quantitative and qualitative improvement of rice. Application of DBC as a screening technique and assessment of Japanese local rice varieties for high protein and lysine. Sci Bull Fac Agric Kyushu Univ 31:145–150
Shiraishi M, Kumamaru T, Satoh H, Omura T, Iwata N, Ogawa M (1986) Variations for storage protein in rice endosperm among varieties. Jpn J Breed (Suppl 2) 36:76–77
Tanaka K, Sugimoto T, Ogawa M, Kasai Z (1980) Isolation and characterization of two types of protein bodies in the rice endosperm. Agric Biol Chem 44:1633–1639
Tanaka Y, Hayashida S, Hongo M (1975 a) Quantitative relation between feces protein particles and rice protein bodies. J Agric Chem Soc Jpn 49:425–429
Tanaka Y, Hayashida S, Hongo M (1975 b) The relationship of the feces protein particles to rice protein bodies. Agric Biol Chem 39:515–518
Wall JS, Fey DA, Paulis LW (1984) Improved two-dimensional electrophoretic separation of zein proteins: Application to study of zein inheritance in corn genotypes. Cereal Chem 61:141–146
Wen TN, Luthe DS (1985) Biochemical characterization of rice glutelin. Plant Physiol 78:172–177
Yamagata H, Tanaka K (1986) The site of synthesis and accumulation of rice storage proteins. Plant Cell Physiol 27:135–145
Yamagata H, Sugimoto T, Tanaka K, Kasai Z (1982) Biosynthesis of storage proteins in developing rice seeds. Plant Physiol 70:1094–1100
Yano M, Okuno K, Kawakami J, Satoh H, Omura T (1985) High amylose mutants of rice, Oryza sativa L. Theor Appl Genet 69:253–257
Yoon KM (1984) Studies on the genetics and breeding of lipid in rice. PhD thesis, Kyushu University, Fukuoka, Japan
Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Routine procedures for growing rice plants in culture solution. In: Laboratory manual for physiological studies of rice. Int Rice Res Inst, Los Baños, Philippines, pp 61–66
Zhao WM, Gatehouse JA, Boulter D (1983) The purification and partial amino acid sequence of a polypeptide from the glutelin fraction of rice grains; homology to pea legumin. FEBS Lett 162:96–102
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Communicated by F. Salamini
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Kumamaru, T., Satoh, H., Iwata, N. et al. Mutants for rice storage proteins. Theoret. Appl. Genetics 76, 11–16 (1988). https://doi.org/10.1007/BF00288825
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DOI: https://doi.org/10.1007/BF00288825