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Improvement of bacterial blight resistance of the popular variety, Nellore Mahsuri (NLR34449) through marker-assisted breeding

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Abstract

To combat the dreaded diseases in rice like bacterial blight (BB) and blast, host plant resistance has been advocated as the most suitable and sustainable method. Through the present study, we have successfully incorporated three major BB resistance genes, namely Xa21, xa13 and xa5 into NLR3449, a high yielding, blast resistant, fine-grain type, popular rice variety through marker-assisted backcross breeding. Foreground selection was carried out using polymerase chain reaction based, gene-specific markers, namely pTA248 (Xa21), xa13prom (xa13) and xa5FM (xa5) at each generation of backcrossing, while 127 polymorphic SSR markers spanning on 12 chromosomes were used for background selection and backcrossing was limited to two rounds. At BC2F1 generation, a single plant (NLR-87-10) with 89.9% recovery, possessing all the three BB resistance genes was forwarded to BC2F2 generation. A solitary BC2F2 plant, namely NLR-87-10-106 possessing all the three resistance genes and 96% genome recovery was identified and advanced through selfing until BC2F4 generation by adopting pedigree-method of selection. Three best BC2F4 lines, possessing high level of resistance against BB and blast, and equivalent or superior to NLR 34449 in terms of yield, grain quality and agro-morphological traits were identified and advanced for multi-location trials.

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Acknowledgements

The first author would like to acknowledge the funding support provided by the M/s Bayer Crop Sciences for necessary support in doing research. The authors would also like to thank Indian Council of Agricultural Research and Department of Biotechnology for meeting part of the funding requirements and thank Project Director, ICAR-IIRR and ANGRAU, Lam, Guntur for providing all the necessary facilities to carry out the research work.

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Author notes

  1. Aleena Dasari and Raman Meenakshi Sundaram contributed equally to this work.

Authors and Affiliations

  1. ICAR-Indian Institute of Rice Research (ICAR-IIRR), Hyderabad, 500 030, India

    Aleena Dasari, Padma Vemulapalli, Rekha Gonuguntla, Dilip Kumar Thota, Punniakotti Elumalai, Kousik Muppavarapu, Swapnil Ravindra Kulkarni, Pragya Sinha, Harika Gunukula, Ravindra Ramarao Kale, Ayyappa Dass Muralidhara, Hajira Shaik, Anila Miriyala, Pranathi Karnati, Mastanbee Shaik, Laha Gouri Shankar, Srinivas Prasad Madamsetty, Balachandran Sena, Gireesh Channappa, Anantha Madhavenkatapura Siddaih, Venkata Subba Rao Lella & Raman Meenakshi Sundaram

  2. Acharya N.G. Ranga Agricultural University (ANGRAU), Lam, Guntur, 522 034, India

    Aleena Dasari, Ratna Babu Didla, Lal Ahamed Mohammad, Venkata Ramana Jagarlamudi & Vijay Gopal Avula

  3. Professor Jayashankar Telangana University (PJTSAU), RARS, Jagitial, 505 529, India

    Laxmi Prasanna Butam

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  1. Aleena Dasari
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  2. Padma Vemulapalli
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Contributions

AD carried out the present work and developed the improved versions of NLR34449 with BB and blast resistance, and drafted the manuscript. PV, BSM, LGS, SLV, SRM provided the infrastructural facilities for developing the improved versions of NLR34449. RMS conceptualized and supervised the experiments and critically reviewed the manuscript. RG constantly helped in developing the improved versions of NLR34449; DT, PE, KM, SRK, SP, HG, KRR, DMA, HSK, LB, AM, PK, MBM LGS, PMS helped with crossings, field selections and data collection for three consecutive seasons. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Raman Meenakshi Sundaram.

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Corresponding editor: Shrish Tiwari

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Dasari, A., Vemulapalli, P., Gonuguntla, R. et al. Improvement of bacterial blight resistance of the popular variety, Nellore Mahsuri (NLR34449) through marker-assisted breeding. J Genet 101, 7 (2022). https://doi.org/10.1007/s12041-021-01340-z

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