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Musa Genetic Diversity Revealed by SRAP and AFLP

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Abstract

The sequence-related amplified polymorphism (SRAP) technique, aimed for the amplification of open reading frames (ORFs), vis-â-vis that of the amplified fragment length polymorphisms (AFLP) were used to analyze the genetic variation and relationships among forty Musa accessions; which include commercial cultivars and wild species of interest for the genetic enhancement of Musa. A total of 403 SRAP and 837 AFLP amplicons were generated by 10 SRAP and 15 AFLP primer combinations, of which 353 and 787 bands were polymorphic, respectively. Both cluster analysis of unweighted pair-grouping method with arithmetic averages (UPGMA) and principal coordinate (PCO) analysis separated the forty accessions into their recognized sections (Eumusa, Australimusa, Callimusa and Rhodochlamys) and species. The percentage of polymorphism amongst sections and species and the relationships within Eumusa species and subspecies varied between the two marker systems. In addition to its practical simplicity, SRAP exhibited approximately threefold more specific and unique bands than AFLP, 37 and 13%, respectively. SRAP markers are demonstrated here to be proficient tools for discriminating amongst M. acuminata, M. balbisiana and M. schizocarpa in the Eumusa section, as well as between plantains and cooking bananas within triploid cultivars.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

ORF:

Open reading frame

SRAP:

Sequence related amplified polymorphism

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Acknowledgments

The authors acknowledge the contributions of MC. Roberto Ku-Cauich, MC. Margarita Aguilar, MC. Adriana Quiroz, IBQ. Jorge Itza and QFB. Miguel Keb for the technical support. This research was funded by the SAGARPA-CONACYT through the research grant 0048160 (RME) and scholarship 811.06.03-6371/07 for the senior author awarded by the government of Mexico through the Ministry of Foreign Affairs.

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  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130 Colonia Chuburná de Hidalgo, 97200, Mérida, Yucatán, México

    Muhammad Youssef, Renata Rivera-Madrid & Rosa María Escobedo-GraciaMedrano

  2. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 # 130 Colonia Chuburná de Hidalgo, 97200, Mérida, Yucatán, México

    Andrew C. James

  3. Martín Napanga 253, Apt. 101, Miraflores, Lima, 18, Perú

    Rodomiro Ortiz

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  1. Muhammad Youssef
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  2. Andrew C. James
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Correspondence to Rosa María Escobedo-GraciaMedrano.

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Youssef, M., James, A.C., Rivera-Madrid, R. et al. Musa Genetic Diversity Revealed by SRAP and AFLP. Mol Biotechnol 47, 189–199 (2011). https://doi.org/10.1007/s12033-010-9328-8

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