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Genetic diversity in an African plantain core collection using AFLP and RAPD markers

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Abstract

Fifteen AFLP primer pairs (EcoRI+3 and MseI+3) and 60 10-mer RAPD primers were used to detect polymorphisms and assess genetic relationships in a sample of 25 plantains from diverse parts of Western and Central Africa. The discriminatory power of the AFLP technique was greater than that of the RAPD technique, since the former produced markers with greater polymorphic information content (PIC) than the latter. Hence, AFLP analysis appeared to be a more-powerful approach for identifying genetic differences among plantain accessions. In this regard, significant genetic diversity within the plantains was shown by the unweighted pair-group method of arithmetic averages (UPGMA) and the multidimensional principal coordinate (PCO) analyses. The AFLP-derived clusters indicated closer relationships between similar inflorescence types than the RAPD-derived clusters. A small group of cultivars from Cameroon were separated from the bulk of other plantains, suggesting that Cameroon may harbour accessions with useful or rare genes for widening the genetic base of breeding populations derived from the plantains. A greater effort should be directed at collecting and characterizing plantain cultivars from Cameroon.

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Acknowledgements

This study was partly funded by the Directorate General for International Cooperation (DGIC), Belgium. This manuscript is no. IITA/62/JA/45.

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Author notes

  1. M. Pillay

    Present address: IITA, Eastern and Southern African Regional Center, P.O. Box 7878, Kampala, Uganda,

Authors and Affiliations

  1. International Institute of Tropical Agriculture, Oyo Road, PMB 5320, Ibadan, Nigeria

    G. Ude, M. Pillay, E. Ogundiwin & A. Tenkouano

  2. IITA, C/o Lambourns UK Ltd, Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, England

    M. Pillay

  3. Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20705, USA

    G. Ude

  4. Department of Biology, California State University, Fresno, CA 93740-8034, USA

    E. Ogundiwin

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  1. G. Ude
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  3. E. Ogundiwin
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  4. A. Tenkouano
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Correspondence to M. Pillay.

Additional information

Communicated by H.F. Linskens

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Ude, G., Pillay, M., Ogundiwin, E. et al. Genetic diversity in an African plantain core collection using AFLP and RAPD markers. Theor Appl Genet 107, 248–255 (2003). https://doi.org/10.1007/s00122-003-1246-8

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  • DOI: https://doi.org/10.1007/s00122-003-1246-8

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