Summary
Taxonomic and phylogenetic determinations within the genus Musa are established using a numerical, morphology-based scoring system. However, within this system, the classification and relationships of some types are disputed. The application of chloroplast DNA (cpDNA) restriction fragment length polymorphism (RFLP) analysis to Musa taxonomy provided valuable, supplemental information about the classification of, and relationships between, Musa species and subspecies. Whole-cell DNA was extracted from lyophilized Musa leaf-blade tissue and digested with various restriction enzymes, Southern blotted onto nylon membranes, and probed using radioactively labeled heterologous orchid cpDNA fragments. Phylogenies were inferred from cpDNA RFLP patterns using PAUP software. The relationships between most species examined were as expected; however, some species (M. beccarii and M. basjoo) did not conform to the conventional morphology-based phylogeny.
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References
Argent CGC (1976) The wild bananas of Papua New Guinea. Notes R Bot Gard Edinburgh 35:77–114
Bonner JW, Warner RM, Brewbaker JL (1974) A chemosystem- atic study of Musa cultivars. HortScience 9 [4]: 325–328
Gawel NJ, Jarret RL (1991) Chloroplast DNA restriction fragment length polymorphisms in Musa germplasm. Euphytica 52:19–23
Jarret RL, Litz R (1986a) Enzyme polymorphism in Musa acuminata Colla. J Hered 77:183–188
Jarret RL, Litz R (1986b) Isozymes as genetic markers in bananas and plantains. Euphytica 35:539–537
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays using tobacco tissue cultures. Physiol Plant 15:473–457
Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Rivera FN (1983) Protein and isoenzyme banding patterns among Philippine cooking bananas and their wild parents (Musa species). Paradisiaca 6:7–12
Rowe PR (1981) Breeding an intractable group. Bananas. In: Rachie KO, Lyman JM (eds) Genetic engineering for crop improvement. Working Papers, The Rockefeller Foundation, New York, pp 66–84
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Shepherd K (1959) Two new basic chromosome numbers in Musaceae. Nature 183:1539
Simmonds NW (1962) The evolution of the bananas. Longman, London
Simmonds NW (1966) Bananas, 2nd edn. Longman, London
Simmonds NW, Shepherd K (1955) The taxonomy and origins of the cultivated bananas. J Linn Soc London Bot 55:302–312
Simmonds NW, Weatherup STC (1990) Numerical taxonomy of the wild bananas (Musa). New Phytol. 115:567–571
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Swofford DL (1985) PAUP-Phylogenetic analysis using parsimony, version 2.4.0. Illinois Natural History Survey, Champaign/IL
Vuylsteke D, Swennen R, Wilson G, De Langhe E (1988) Phenotypic variation among in vitro-propagated plantain (Musa sp. cultivar ‘AAB’). Sci Hortic 36:79–88
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Communicated by G. Wenzel
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Gawel, N.J., Jarret, R.L. Chloroplast DNA restriction fragment length polymorphisms (RFLPs) in Musa species. Theoret. Appl. Genetics 81, 783–786 (1991). https://doi.org/10.1007/BF00224990
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DOI: https://doi.org/10.1007/BF00224990