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Improved Production of Raw Starch Degrading Enzyme by Aspergillus oryzae F-30 Using Methyl Glucoside Sesqui-Stearate

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Abstract

The effect of methyl glucoside sesqui-stearate (MGS) on the production of raw starch degrading enzyme (RSDE) by Aspergillus oryzae F-30 was studied in this paper. The activity of RSDE formed by Aspergillus oryzae F-30 was enhanced dramatically by the addition of MGS to the medium. As a result, with the addition of 1.5 g MGS in 1 L basal medium, RSDE activity and productivity were 107 U mL−1 and 1.49 U mL−1 h−1, 4.3-fold and 7.1-fold greater than the values obtained in the basal medium, respectively. The effect of MGS on the synthesis of RSDE by Aspergillus oryzae F-30 was also studied on a molecular level. It was observed that the regulation of RSDE synthesis in Aspergillus oryzae F-30 occurs at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of MGS at transcriptional level.

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Acknowledgements

This research was supported by Chinese 863 Project (no. 2007AA021307).

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Authors and Affiliations

  1. State Key Laboratory of Tropical Corp Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571101, China

    Hai-Yan Sun & Ming Peng

  2. Wuxi Scientific Research and Designing institute of the State Administration of Grain Reserve, Wuxi, Jiangsu Province, 214036, China

    Lu Wang

  3. Research Center of Enzymes, AsiaPac Bio-technology Co. Ltd., Dongguan, Guangdong Province, 523808, China

    Jian-Wen Liu

Authors
  1. Hai-Yan Sun
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  2. Lu Wang
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  3. Jian-Wen Liu
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  4. Ming Peng
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Corresponding authors

Correspondence to Jian-Wen Liu or Ming Peng.

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Sun, HY., Wang, L., Liu, JW. et al. Improved Production of Raw Starch Degrading Enzyme by Aspergillus oryzae F-30 Using Methyl Glucoside Sesqui-Stearate. Appl Biochem Biotechnol 159, 78–84 (2009). https://doi.org/10.1007/s12010-008-8413-y

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  • DOI: https://doi.org/10.1007/s12010-008-8413-y

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