Summary
The specificity of induction of wooddegrading enzymes from Pullularia pullulans was investigated using series of mono-, di- and (1→4)-β-trisaccharides or glycanes. A strain of P. pullulans (1740), unable to grow on Avicel or carboxymethyl-cellulose (CMC), uses xylan and steamexploded wood as carbon sources. This strain, thus grown, was evaluated for various enzyme activities. d-Xylose was the nutritional inducer of β-xylosidase and β-xylanase. d-Glucuronic acid induced activity on CMC and β-glucosidase activity was observed regardless of carbon source used. (1→4)-β-Xylobiose was not an inducer of β-xylanase production, but high levels of this enzyme were obtained with either structural isomers (1→2)β or (1→3)-β. Since synthesis of this enzyme was stimulated by increasing xylose concentration yp to 40 g/l, it is suggested that xylose enters the cells by passive transport and is unable to induce a permease system.
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Affiliated to the Scientific, Technological and Medical University of Grenoble
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Pou-Llinas, J., Driguez, H. d-Xylose as inducer of the xylan-degrading enzyme system in the yeast Pullularia pullulans . Appl Microbiol Biotechnol 27, 134–138 (1987). https://doi.org/10.1007/BF00251935
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DOI: https://doi.org/10.1007/BF00251935