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Production of raw-starch-hydrolysing α-amylase from the newly isolated Geobacillus thermodenitrificans HRO10

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Abstract

An extracellular raw-starch-digesting α-amylase was isolated from Geobacillus thermodenitrificans HRO10. The culture conditions for the production of α-amylase by G. thermodenitrificans HRO10 was optimized in 1.2–l bioreactor using full 24 and 32 factorial designs. From the optimal reaction conditions, a model (Y = − 594.206 − 0.178T2 − 8.448pH2 + 6.020TpH − 0.005T2pH2) was predicted, which was then used for α-amylase production. In the bioreactor studies, the enzyme yield under optimized conditions (pH 7.1, 49°C) was 30.20 U/ml, a 51% improvement over the results (19.97 U/ml) obtained when the traditional one-factor-at-a-time method was employed. This α-amylase does not require extraneous calcium ions for activity, which may be a commercially important observation.

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Acknowledgements

This research work was funded through a fellowship from the German Academic Exchange Service (DAAD) to Thaddeus Ezeji and by the Fonds der Chemischen Industrie. We thank Hella Goschke, Ilona Boldt, and Arite Wolf for their excellent technical assistance; and Dr. (Mrs.) Marie Hubert for kindly assisting us with SPSS analysis.

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  1. Thaddeus C. Ezeji

    Present address: Department of Food Science and Human Nutrition, University of Illinois, Biotechnology And Bioengineering Group, Room 496 ASL, 1207 W Gregory Drive, Urbana, IL, 61801, USA

Authors and Affiliations

  1. Institute of Biological Sciences, Division of Microbiology, University of Rostock, Albert-Einstein-Str. 3, 19051, Rostock, Germany

    Thaddeus C. Ezeji & Hubert Bahl

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  1. Thaddeus C. Ezeji
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  2. Hubert Bahl
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Correspondence to Thaddeus C. Ezeji.

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Ezeji, T.C., Bahl, H. Production of raw-starch-hydrolysing α-amylase from the newly isolated Geobacillus thermodenitrificans HRO10. World J Microbiol Biotechnol 23, 1311–1315 (2007). https://doi.org/10.1007/s11274-007-9353-3

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  • DOI: https://doi.org/10.1007/s11274-007-9353-3

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