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Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of Lactarius deliciosus

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Abstract

Fungi play an important role in dying wastewater treatment. In this work, the mycelia of Lactarius deliciosus exhibited an excellent capacity in decolorizing coomassie brilliant blue (CBB). The results demonstrated that the mycelia could treat CBB with high concentrations over a broad range of pH and temperature. The decolorization rate of 99.19% and the removal rate of 16.31 mg·L−1·h were realized. The mycelia could be recycled from decolorizing process for 19 times, indicating a good re-usability. It verified that the lignin peroxidase (121.65 U·L−1) and manganese peroxidase (36.77 U·L−1) were involved in the degradation and decolorization process of CBB. Toxicity assessments indicated the seed germination rate was up to 82.22% while inhibition to Escherichia coli decreased dramatically and no significant effect on Caenorhabditis elegans growth was found. The removal of CBB was a synergistic process accomplished by adsorption and biodegradation. The mycelia could be used for eco-friendly CBB treatment.

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Acknowledgements

This work was supported by the Anhui Provincial Program on Key Research and Development Project (Grant No. 202004a06020021), the National Natural Science Foundation of China (Grant No. 21606002), the Natural Science Foundation of Anhui Province (CN) (Grant No. 1708085QC64) and the Undergraduate Research Training Programs for Innovation (Grant Nos. 201910357069, S201910357427).

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Authors and Affiliations

  1. School of Life Sciences, Anhui University, Hefei, 230601, China

    Jin Zhao, Qing-Xi Wu, Xiao-Du Cheng, Ting Su, Xiao-Hui Wang, Wen-Na Zhang, Yong-Ming Lu & Yan Chen

  2. Anhui Key Laboratory of Modern Biomanufacturing, Hefei, 230601, China

    Qing-Xi Wu, Wen-Na Zhang, Yong-Ming Lu & Yan Chen

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  1. Jin Zhao
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  2. Qing-Xi Wu
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  3. Xiao-Du Cheng
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Correspondence to Qing-Xi Wu or Yan Chen.

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11705_2020_1952_MOESM1_ESM.pdf

Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of Lactarius deliciosus

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Zhao, J., Wu, QX., Cheng, XD. et al. Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of Lactarius deliciosus. Front. Chem. Sci. Eng. 15, 421–436 (2021). https://doi.org/10.1007/s11705-020-1952-7

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