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Extracellular Enzymatic Activities of Bacterial Strains Isolated from Tunisian Biotopes: Decolorization and Detoxification of Indigo Carmine

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Abstract

Eleven bacterial strains, isolated from various Tunisian biotopes, were characterized for their lignin degradation activities and were screened for Indigo Carmine (IC) decolorization for the first time. Results revealed that these isolated strains were able to decolorize IC. Based on this property, the newly isolates crude filtrates (NICF) showed that eight ones prove the effectiveness for IC degradation (100 mg). More than 80% of IC could be decolorized within 12 h under non-optimised conditions. The UV–Visible absorption spectra and FT-IR analysis of the degradation products showed an IC decolorization and/or transformation, proving the enzyme’s role in dye decolorization. Furthermore, the phytotoxicity and the cytotoxicity using Vero and erythrocyte cells were performed to evaluate the acute toxicity of the treated and untreated dye. The results showed that some NICF can decrease and sometimes detoxify IC. In fact, NICF decolorize the toxic IC into non-toxic products. These NICF, acting as powerful tools, could be effectively used to decolorize and to bioremediate rich-dye-textile effluents and found worthy of investigation for potential applications in restoration work and other biotechnological uses.

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Authors and Affiliations

  1. Faculté Des Sciences de Gafsa, Université de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, 2112, Gafsa, Tunisia

    Sonia Ben Younes, Chadia Dallali & Ali Ellafi

  2. Laboratoire Des Bioprocédés Environnementaux, Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour Km 6, BP “1177”, 3018, Sfax, Tunisia

    Sonia Ben Younes

  3. Center of Biotechnology of Borj Cedria, LR15 CBBC03 Bioactive Substances, University of Tunis El Manar, 2050, Tunis, Tunisia

    Lamjed Bouslama

  4. Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, Gafsa, Tunisia

    Anwar Feriani

  5. Center for Sustainable Development, College of Arts and Sciences, Qatar University, 2713, Doha, Qatar

    Sami Sayadi

Authors
  1. Sonia Ben Younes
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  2. Chadia Dallali
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  3. Ali Ellafi
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  4. Lamjed Bouslama
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  5. Anwar Feriani
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  6. Sami Sayadi
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Correspondence to Sonia Ben Younes or Sami Sayadi.

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Ben Younes, S., Dallali, C., Ellafi, A. et al. Extracellular Enzymatic Activities of Bacterial Strains Isolated from Tunisian Biotopes: Decolorization and Detoxification of Indigo Carmine. Catal Lett 151, 1248–1261 (2021). https://doi.org/10.1007/s10562-020-03405-7

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  • DOI: https://doi.org/10.1007/s10562-020-03405-7

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