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Phytoremediation potential of Petunia grandiflora Juss., an ornamental plant to degrade a disperse, disulfonated triphenylmethane textile dye Brilliant Blue G

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Abstract

Phytoremediation provides an ecofriendly alternative for the treatment of pollutants like textile dyes. The purpose of this study was to explore phytoremediation potential of Petunia grandiflora Juss. by using its wild as well as tissue-cultured plantlets to decolorize Brilliant Blue G (BBG) dye, a sample of dye mixture and a real textile effluent. In vitro cultures of P. grandiflora were obtained by seed culture method. The decolorization experiments were carried out using wild as well as tissue-cultured plants independently. The enzymatic analysis of the plant roots was performed before and after decolorization of BBG. Metabolites formed after dye degradation were analyzed using UV–vis spectroscopy, high-performance liquid chromatography, Fourier transform infrared spectroscopy, and gas chromatography–mass spectrometry. Phytotoxicity studies were performed. Characterization of dye mixture and textile effluent was also studied. The wild and tissue-cultured plants of P. grandiflora showed the decolorized BBG up to 86 %. Significant increase in the activities of lignin peroxidase, laccase, NADH-2,6-dichlorophenol-indophenol reductase, and tyrosinase was found in the roots of the plants. Three metabolites of BBG were identified as 3-{[ethyl(phenyl)amino]methyl}benzenesulfonic acid, 3-{[methyl (phenyl)amino]methyl}benzenesulfonic amino acid, and sodium-3-[(cyclohexa-2,5-dien-1-ylideneamino)methyl]benzenesulfonate. Textile effluent sample and a synthetic mixture of dyes were also decolorized by P. grandiflora. Phytotoxicity test revealed the nontoxic nature of metabolites. P. grandiflora showed the potential to decolorize and degrade BBG to nontoxic metabolites. The plant has efficiently treated a sample of dye mixture and textile effluent.

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Authors and Affiliations

  1. Department of Biotechnology, Shivaji University, Kolhapur, 416 004, India

    Anuprita D. Watharkar, Rahul V. Khandare, Apurva A. Kamble & Jyoti P. Jadhav

  2. Department of Biochemistry, Shivaji University, Kolhapur, India

    Asma Y. Mulla & Sanjay P. Govindwar

Authors
  1. Anuprita D. Watharkar
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  2. Rahul V. Khandare
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  3. Apurva A. Kamble
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  4. Asma Y. Mulla
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  5. Sanjay P. Govindwar
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  6. Jyoti P. Jadhav
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Correspondence to Jyoti P. Jadhav.

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Watharkar, A.D., Khandare, R.V., Kamble, A.A. et al. Phytoremediation potential of Petunia grandiflora Juss., an ornamental plant to degrade a disperse, disulfonated triphenylmethane textile dye Brilliant Blue G. Environ Sci Pollut Res 20, 939–949 (2013). https://doi.org/10.1007/s11356-012-0904-2

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