Abstract
The effect of β-lactam antibiotics viz. cefotaxime, carbenicillin and cephalexin was evaluated on shoot organogenesis of potato cultivars ‘Kufri Pukhraj’ and ‘Kufri Chipsona 1’. Leaf and internodal explants from 21-day-old microshoots were cultured on pre-standardized medium for shoot organogenesis (basal Murashige and Skoog (MS) medium containing 10 μM AgNO3+10 μM 6-benzyladenine (BA)+15 μM gibberellin A3 (GA3)), which was further supplemented with different concentrations (0–500 mg/L) of various β-lactam antibiotics. At lower concentrations, these antibiotics promoted shoot organogenesis in both cultivars, except cephalexin that totally inhibited shoot organogenesis in cv. ‘Kufri Chipsona 1’. Cefotaxime (100 mg/L) induced shoot organogenesis in maximum number of explants with maximum number of shoots per explant. Among explants, shoot promotion effect was significantly higher from internodal explants as compared with leaf explants. Cefotaxime at all concentrations (100–500 mg/L) increased shoot organogenesis of all explants and cultivars. The regenerated shoots tested clonally uniform among themselves and also to that of the mother plant by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers.
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References
Abbasi AZ, Bilal M, Hussain J, Shah MM, Hassan A, Kwon S, Ur-Rehman S, Ahmad R (2016) Robust regeneration protocol for the Agrobacterium tumefaciens mediated transformation of Solanum tuberosum. Pak J Bot 48:707–712
Abd Elaleem KG, Modawai RS, Khalafalla MM (2009) Effect of plant growth regulators on callus induction and plant regeneration in tuber segment culture of potato (Solanum tuberosum L.) cultivar Diamant. Afr J Biotechnol 8:2529–2534. https://doi.org/10.5897/AJB09.014
Aggarwal D, Kumar A, Reddy MS (2010) Shoot organogenesis in elite clones of Eucalyptus tereticornis. Plant Cell Tiss Organ Cult 102:45–52. https://doi.org/10.1007/s11240-010-9703-y
Aggarwal D, Kumar A, Reddy MS (2011) Agrobacterium tumefaciens mediated genetic transformation of selected elite clone(s) of Eucalyptus tereticornis. Acta Physiol Plant 33:1603–1611. https://doi.org/10.1007/s11738-010-0695-3
Ahmad MZ, Hussain I, Muhammad A, Ali S, Ali GM, Roomi S, Zia MA, Ijaz A (2012) Factor affecting Agrobacterium-mediated transformation of rice chitinase gene in (Solanum tuberosum L). Afr J Biotechnol 11:9716–9723. https://doi.org/10.5897/AJB11.1961
Aoshima Y (2005) Efficient embryogenesis in the callus of tea (Camellia sinensis) enhanced by osmotic stress or antibiotics treatment. Plant Biotechnol 22:277–280. https://doi.org/10.5511/plantbiotechnology.22.277
Borrelli GM, Di Fonzo N, Lupotto E (1992) Effect of cefotaxime on callus culture and plant regeneration in durum wheat. J Plant Physiol 140:372–374. https://doi.org/10.1016/S0176-1617:81095-3
Bosela MJ (2009) Effect of β-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides x P. tremula and P. x canescens x P. grandidentata. Plant Cell Tiss Organ Cult 98:249–261. https://doi.org/10.1007/s11240-009-9555-5
Chuntale K (2018) Biotechnological approaches to improve potato: review article. Food Science and Quality Management 77:15–23
Costa MGC, Nogueira FTS, Figueira ML, Otoni WC, Brommonschenkel SH, Cecon PR (2000) Influence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep 19:327–332. https://doi.org/10.1007/s002990050021
da Silva Mendes AF, Cidale LC, de Oliveira MLP, Otoni WC, Soares-Filho WDS, Costa MGC (2009) Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb. Plant Cell Tiss Organ Cult 97:331–336. https://doi.org/10.1007/s11240-009-9518-x
Dai W, Castillo C (2007) Factors affecting plant regeneration from leaf tissues of Buddleia species. Hortscience 42:1670–1673. https://doi.org/10.21273/HORTSCI.42.7.1670
Ding X, Chen X, Li W, Du Z (2006) Effects of antibiotics on plantlet regeneration via organogenesis in Populus euphratica. For Stud China 8:27–31. https://doi.org/10.1007/s11632-006-0005-8
Doyle JJ, Doyle JL (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13–15
Estopa M, Marfa V, Mele E, Messeguer J (2001) Study of different antibiotic combinations for use in the elimination of Agrobacterium with kanamycin selection in carnation. Plant Cell Tiss Organ Cult 65:211–220. https://doi.org/10.1023/A:1010630726444
FAOSTAT (2018) Food AND Agricultural Organization of the United Nations (http://www.fao.org/faostat/en/#data/QC). Accessed 26 Sept 2020
Gerszberg A, Grzegorczyk-Karolak I (2019) Influence of selected antibiotics on the tomato regeneration in in vitro cultures. Not Bot Horti Agrobo 47:558–564. https://doi.org/10.15835/nbha47311401
Ghosh S, Majumdar S, Sarkar D, Datta K (2014) An efficient adventitious shoot regeneration system for potato (Solanum tuberosum L.) using leaf discs. J Plant Biochem Biotechnol 24:298–304. https://doi.org/10.1007/s13562-014-0273-7
Grafi G (2004) How cells differentiate: a lesson from plants. Dev Biol 236:1–6. https://doi.org/10.1016/j.ydbio.2003.12.027
Grewal D, Gill R, Gosal SS (2006) Influence of antibiotic cefotaxime on somatic embryogenesis and plant regeneration in indica rice. Biotechnol J 1:1158–1162. https://doi.org/10.1002/biot.200600139
Grzebelus E, Skop L (2014) Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures. In Vitro Cell Dev Biol Plant 50:568–575. https://doi.org/10.1007/s11627-014-9626-0
Haddadi F, Aziz MA, Abdullah SNA, Tan SG, Kamaladini H (2015) An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by a dual plasmid system. Molecules 20:3647–3666. https://doi.org/10.3390/molecules20033647
Halterman D, Guenthner J, Collinge S, Butler N, Douches D (2016) Biotech potatoes in the 21st century: 20 years since the first biotech potato. Am J Potato Res 90:1–20. https://doi.org/10.1007/s12230-015-9485-1
Ibrahim IA, Emara HA, Nower AA, Abodiab AY (2017) In vitro cultivation of potato plants. Int J Curr Microbiol Appl Sci 5:858–868. https://doi.org/10.20546/ijcmas.2016.512.094
Ijaz S, Idrees S, Khan TM (2016) Protocol optimization for in vitro regeneration system in frost sensitive potato genotypes: as a basis for genetic transformation studies in Solanum tuberosum L. Plant Physiol Biochem 4:169. https://doi.org/10.4172/23299029.1000169
Kaur A, Gill M, Ruma D, Gosal S (2008) Enhanced in vitro shoot multiplication and elongation in sugarcane using cefotaxime. Sugar Tech 10:60–64. https://doi.org/10.1007/s12355-008-0010-4
Kaur A, Guleria S, Reddy MS, Kumar A (2020) A robust genetic transformation protocol to obtain transgenic shoots of Solanum tuberosum L. cultivar ‘Kufri Chipsona 1’. Physiol Mol Biol Plants 26:367–377. https://doi.org/10.1007/s12298-019-00747-4
Kaur A, Reddy MS, Kumar A (2017) Efficient one step and cultivar independent shoot organogenesis of potato. Physiol Mol Biol Plants 23:461–469. https://doi.org/10.1007/s12298-017-0418-y
Kumar A, Aggarwal D, Gupta P, Reddy MS (2010) Factor affecting in vitro propagation and field establishment of Chlorophytum borivilianum. Biol Plant 54:601–606. https://doi.org/10.1007/s10535-010-0109-9
Kumlay AM, Ercisli S (2015) Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosum L.) stem node and leaf explants under long-day conditions. Biotechnol Biotechnol Equip 29:1075–1084. https://doi.org/10.1080/13102818.2015.1077685
Mahadev MD, Panathila CS, Naidu CV (2014) Influence of bavistin, cefotaxime, kanamycin and silver thiosulphate on plant regeneration of Solanum viarum (Dunal) - an important anticancer medicinal plant. Am J Plant Sci 5:403–408. https://doi.org/10.4236/AJPS.2014.53053
Meng Q, Liu Z, Zhang Y, Liu C, Ren F, Feng H (2014) Effects of antibiotics on in vitro-cultured cotyledons. In Vitro Cell Dev Biol-Plant 50:436–441. https://doi.org/10.1007/s11627-014-9595-3
Mittal P, Gosal SS, Senger A, Kumar P (2009) Impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane. Physiol Mol Biol Plants 15:257–265. https://doi.org/10.1007/s12298-009-0029-3
Naing AH, Park KI, Lim SH, Kim CHK (2014) Appropriate choice of antibiotics for plant regeneration and optimization of selective agents to be used in genetic transformation of chrysanthemum. Plant Omics 7:237–243
Nauerbey B, Billing K, Wyndaele R (1997) Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci 123:169–177. https://doi.org/10.1016/S0168-9452(96)04569-4
Predieri S, Malavasi FFF, Passey AJ, Ridout MS, James DJ (1989) Regeneration from in-vitro leaves of ‘Conference’ and other pear cultivars (Pyrus communis L.). J Hortic Sci 64:553–559. https://doi.org/10.1080/14620316.1989.1151599
Qin YH, Teixeira da Silva JA, Bi JH, Zhang SL, Hu GB (2011) Response of in vitro strawberry to antibiotics. Plant Growth Regul 65:183–193. https://doi.org/10.1007/s10725-0119587-9
Rani V, Raina SN (1998) Genetic analysis of enhanced-axillary branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. Plants. Plant Cell Rep 17:236–242. https://doi.org/10.1007/s002990050385
Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MJ (2015) Antibiotic resistance and extended spectrum beta-lactamases: types, epidemiology and treatment. Saudi J Biol Sci 22:90–101. https://doi.org/10.1016/j.sjbs.2014.08.002
Souza BM, Kraus JE, Endres L, Mercier H (2003) Relationships between endogenous hormonal levels and axillary bud development of Ananas comosus nodal segments. Plant Physiol Biochem 41:733–739. https://doi.org/10.1016/S0981-9428(03)00112-8
Stanisic M, Ninkovic S, Savic J, Cosic T, Mitic N (2018) The effects of β-lactam antibiotics and hygromycin B on de nova shoot organogenesis in apple cv. Golden Delicious. Arch Biol Sci 70:179–190. https://doi.org/10.2298/ABS170731037S
Su Y, Liu Y, Zhang X (2011) Auxin-Cytokinin interaction regulates meristem development. Mol Plant 4:616–625. https://doi.org/10.1093/mp/ssr007
Tambarussi EV, Rogalski M, Nogueira FTS, Brondani GE, De Martin V, Carrer H (2015) Influence of antibiotics on indirect organogenesis of Teak. Ann For Res 58:177–183. https://doi.org/10.15287/afr.2015.345
Tereso S, Miguel C, Maroco J, Oliveira MM (2006) Susceptibility of embryogenic and organogenic tissues of maritime pine (Pinus pinaster) to antibiotics used in Agrobacterium-mediated genetic transformation. Plant Cell Tiss Organ Cult 87:33–40. https://doi.org/10.1007/s11240-006-9130-2
Tiwari NN, Singh RK, Singh SP (2018) Impact of cefotaxime on Agrobacterium mediated BTgene transformation in sugarcane. J Pharmacogn Phytochem 7:3952–3955
Torregorsa L, Peros JP, Lopaz G, Bouquet A (2000) Effect of hygromycin, kanamycin and phosphinothricin on the embryogenic callus development and axillary micropropagation of Vitis vinifera L. Acta Hortic 528:401–406. https://doi.org/10.17660/ActaHortic.2000.528.59
Tran TN, Sanan-Mishra N (2015) Effect of antibiotics on callus regeneration during transformation of IR 64 rice. Biotechnol Rep 7:143–149. https://doi.org/10.1016/j.btre.2015.06.004
Wei T, Harris L, Newton RJ (2003) Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.). J For Res 14:185–190. https://doi.org/10.1007/BF02856827
Wiebke B, Ferreira F, Pasquali G, Bodanese-Zanettini MH, Droste A (2006) Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation. Bragantia 65:543–551. https://doi.org/10.1590/S000687052006000400002
Yepes LM, Aldwinckle HS (1994) Factors that affect leaf regeneration efficiency in apple and effect of antibiotics in morphogenesis. Plant Cell Tiss Organ Cult 37:257–269. https://doi.org/10.1007/BF00042339
Yu TA, Yeh SD, Yang JS (2001) Effect of carbenicillin and cefotaxime on callus growth and somatic embryogenesis from adventitious roots of papaya. Bot Bull Acad Sin 42:281–228. https://doi.org/10.7016/BBAS.200110.0281
Zhang H, Xu F, Wu Y, Hu HH, Dai XF (2017) Progress of potato staple food research and industry development in China. J Integr Agric 16:2924–2932. https://doi.org/10.1016/S2095-3119(17)61736-2
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The author thankfully acknowledges TIFAC-CORE, Thapar Institute of Engineering and Technology for providing facilities and necessary help to carry out this research work.
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The authors thank the Council of Scientific and Industrial Research, Extramural Research Division, New Delhi, for funding this study through the project (sanction no. 38(1465)/18/EMR-II).
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AK designed experiments, and SG conducted experiments, analysed data, and compiled the first draft of manuscript. AK finalized manuscript and submitted to journal.
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Guleria, S., Kumar, A. Enhancement of Shoot Organogenesis of Potato Cultivars ‘Kufri Pukhraj’ and ‘Kufri Chipsona 1’ by β-lactam Antibiotics. Potato Res. 65, 137–151 (2022). https://doi.org/10.1007/s11540-021-09513-0
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DOI: https://doi.org/10.1007/s11540-021-09513-0