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Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116–290 mM in the basal medium (BM) promoted shoot multiplication. Heat shock (50 °C, 1 h) also promoted shoot multiplication at these sucrose concentrations on both BM medium and BM supplemented with 5.0 μM BA. Beneficial effect of sucrose was also observed on rooting of shoots on BM as well as BM supplemented with 5.0 μM indole-3-butyric acid (IBA). Phytagel as a gelling agent was found to be more effective for shoot proliferation and growth compared to agar. Amongst various soil mixtures tested, higher survival of plants was observed in soil containing Vermicompost. It was interesting to note that a maximum plant survival (> 95 %) was observed when plants were directly transferred to net-house (irradiance reduced to 50 % with green net, without humidity and temperature control) than poly-house (with humidity and temperature control). Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis of regenerated plants showed genetic similarity to mother plant.

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Abbreviations

BA:

benzyladenine

BAM:

basal MS medium supplemented with 5.0 μM BA

BIBA:

basal MS medium supplemented with 5.0 μM IBA

BM:

basal MS medium

HS:

heat shock

IBA:

indole-3-butyric acid

ISSR:

inter simple sequence repeat

KIN:

kinetin

PGRs:

plant growth regulators

PCR:

polymerase chain reaction

RAPD:

random amplified polymorphic DNA

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Acknowledgement

Authors are thankful to TIFAC-CORE, Thapar University Patiala for the facilities to carry out this work.

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Authors and Affiliations

  1. Centre of Relevance and Excellence in Agro and Industrial Biotechnology, Thapar University, Patiala, 147004, Punjab, India

    A. Kumar, D. Aggarwal, P. Gupta & M. S. Reddy

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  1. A. Kumar
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  2. D. Aggarwal
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  3. P. Gupta
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  4. M. S. Reddy
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Correspondence to A. Kumar.

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Kumar, A., Aggarwal, D., Gupta, P. et al. Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum . Biol Plant 54, 601–606 (2010). https://doi.org/10.1007/s10535-010-0109-9

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  • DOI: https://doi.org/10.1007/s10535-010-0109-9

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