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Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb.

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Abstract

Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sinensis, in comparison with the commonly used beta-lactam cefotaxime. It was observed that, in contrast to cefotaxime, meropenem and timentin had a positive or no detrimental effect on the shoot regeneration from epicotyl explants. Residual effects of the beta-lactams from shoot regeneration medium also affected the subsequent ability of the roots to elongate. The addition of meropenem and/or timentin in the rooting medium mostly improved or did not affect the rooting ability of the adventitious shoots. These data indicated that meropenem and timentin can positively replace cefotaxime in Agrobacterium-mediated transformation of C. sinensis.

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Abbreviations

BAP:

6-Benzylaminopurine

IBA:

Indolebutyric acid

MS:

Murashige and Skoog

NAA:

Naphtaleneacetic acid

RIM:

Rooting medium

SIM:

Shoot induction medium

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Acknowledgments

To International Foundation for Science (IFS) for financial support; to The State of Bahia Research Foundation (FAPESB), for financial and fellowship support; to National Council for Scientific and Technological Development (CNPq) and Coordination of Higher Education and Graduate Training (CAPES), for fellowships support.

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Authors and Affiliations

  1. Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rod. Ilhéus-Itabuna km 16, Ilhéus, Bahia, 45662-000, Brazil

    Amanda Ferreira da Silva Mendes, Luciana Cardoso Cidade & Marcio Gilberto Cardoso Costa

  2. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil

    Maria Luiza Peixoto de Oliveira & Wagner Campos Otoni

  3. Embrapa Mandioca e Fruticultura Tropical, Cruz das Almas, Bahia, 44380-000, Brazil

    Walter Dos Santos Soares-Filho

Authors
  1. Amanda Ferreira da Silva Mendes
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  2. Luciana Cardoso Cidade
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  3. Maria Luiza Peixoto de Oliveira
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  4. Wagner Campos Otoni
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  5. Walter Dos Santos Soares-Filho
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  6. Marcio Gilberto Cardoso Costa
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Correspondence to Marcio Gilberto Cardoso Costa.

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da Silva Mendes, A.F., Cidade, L.C., de Oliveira, M.L.P. et al. Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb.. Plant Cell Tiss Organ Cult 97, 331–336 (2009). https://doi.org/10.1007/s11240-009-9518-x

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  • DOI: https://doi.org/10.1007/s11240-009-9518-x

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