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Identification of S-genotypes in Chinese cherry cultivars (Prunus pseudocerasus Lindl.)

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Abstract

Self-incompatibility has been studied extensively at the molecular level in Solanaceae, Rosaceae, and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility. In the present study, we successfully isolated nine S-RNase alleles from cultivars of Chinese cherry by PCR amplification from genomic DNA and stylar cDNA combining with cleaved amplified polymorphic sequence marker. Analysis of amino acid sequences revealed five novel S-alleles, S 2 , S 4 , S 6 , S 8 , and S 9 , with respective accession numbers in the NCBI database of EF541168, EF541173, EF541172, FJ628598, and FJ628599. Results showed that “Dongtang” and “Yinzhu” contained six S-alleles (S 1 , S 3 , S 5 , S 7 , S 8 , and S 9 ); “Taishanganying” contained four S-alleles (S 1 , S 2 , S 4 , and S 6 ); “Daiba”, “Dayingzui”, and “Xiaomizi” contained four S-alleles (S 1 , S 2 , S 5 , and S 8 ); “Laiyangduanzhi”, “Shuangquanchangba”, and “Daqingye” contained three S-alleles (S 1 , S 2 , and S 8 ). It is interesting that different cultivars collected from the same place hold the same S-genotypes. Moreover, pollination tests and pollen tube growth assays showed that nine cultivars were self-compatible. Chinese cherry presented in this article are naturally polyploidy, which is a very useful material for the study of self-compatibility, and much of this information will be valuable for further work on self-(in)compatibility of fruit tree in Rosaceae.

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Abbreviations

Pps :

Prunus pseudocerasus

Pa :

Prunus avium

Par :

Prunus armeniaca

Pd :

Prunus dulcis

Pm :

Prunus mume

Ps :

Prunus salicina

Pspe :

Prunus speciosa

Pspi :

Prunus spinosa

Pb :

Pyrus × bretschneideri

Pc :

Pyrus communis

Ppy :

Pyrus pyrifolia

Pu :

Pyrus ussuriensis

Md :

Malus × domestica

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Authors and Affiliations

  1. College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People’s Republic of China

    Chao Gu, Shao-Ling Zhang, Shao-Xi Huang, Wei Heng, Hua-Qing Wu & Jun Wu

  2. Key Laboratory for Fruit Biotechnology Breeding of Shandong, Pomological Institute of Shandong, Taian, Shandong, 271000, People’s Republic of China

    Qing-Zhong Liu

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  1. Chao Gu
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  2. Shao-Ling Zhang
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  3. Shao-Xi Huang
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  4. Wei Heng
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  5. Qing-Zhong Liu
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  6. Hua-Qing Wu
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  7. Jun Wu
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Correspondence to Jun Wu.

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Communicated by A. Abbott

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Gu, C., Zhang, SL., Huang, SX. et al. Identification of S-genotypes in Chinese cherry cultivars (Prunus pseudocerasus Lindl.). Tree Genetics & Genomes 6, 579–590 (2010). https://doi.org/10.1007/s11295-010-0273-2

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