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Inheritance and interactions of incompatibility alleles in the tetraploid sour cherry

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Abstract

Three progenies of sour cherry (Prunus cerasus) were analysed to correlate self-(in)compatibility status with S-RNase phenotype in this allotetraploid hybrid of sweet and ground cherry. Self-(in)compatibility was assessed in the field and by monitoring pollen tube growth after selfing. The S-RNase phenotypes were determined by isoelectric focusing of stylar proteins and staining for RNase activity and, for the parents, confirmed by PCR. Seedling phenotypes were generally consistent with disomic segregation of S-RNase alleles. The genetic arrangements of the parents were deduced to be ‘Köröser’ (self-incompatible) S 1 S 4 .S B S D , ‘Schattenmorelle’ (self-compatible) S 6 S 13 .S B S B , and clone 43.87 (self-compatible) S 4 S 13 .S B S B , where “.” separates the two homoeologous genomes. The presence of S 4 and S 6 alleles at the same locus led to self-incompatibility, whereas S 13 and S B at homoeologous loci led to self-compatibility. The failure of certain heteroallelic genotypes in the three crosses or in the self-incompatible seedlings indicates that S 4 and S 6 are dominant to S B . However, the success of S 13 S B pollen on styles expressing corresponding S-RNases indicates competitive interaction or lack of pollen-S components. In general, the universal compatibility of S 13 S B pollen may explain the frequent occurrence of S 13 and S B together in sour cherry cultivars. Alleles S B and S D , that are presumed to derive from ground cherry, and S 13 , presumably from sweet cherry, were sequenced. Our findings contribute to an understanding of inheritance of self-(in)compatibility, facilitate screening of progenies for self-compatibility and provide a basis for studying molecular interactions in heteroallelic pollen.

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Acknowledgements

Cherry genetics at East Malling is supported by the Department for Environment, Food and Rural Affairs. Radovan Bošković acknowledges a grant from the Mount Trust and Tineke Sonneveld, a studentship from Horticulture Research International and University of Nottingham.

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Authors and Affiliations

  1. East Malling Research, New Road, East Malling, Kent, ME19 6BJ, UK

    R.I. Bošković, K.R. Tobutt & T. Sonneveld

  2. Imperial College at Wye, Ashford, Kent, TN25 5AH, UK

    R.I. Bošković

  3. Institut für Obstzüchtung, Pillnitzer Platz 2, 01326, Dresden, Germany

    B. Wolfram

  4. Fruit and Grape Research Centre, Kralja Petra I/9, 32000, Čačak, Serbia and Montenegro

    R. Cerović

Authors
  1. R.I. Bošković
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  2. B. Wolfram
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  3. K.R. Tobutt
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  4. R. Cerović
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  5. T. Sonneveld
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Correspondence to R.I. Bošković.

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Communicated by H. Nybom

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Bošković, R., Wolfram, B., Tobutt, K. et al. Inheritance and interactions of incompatibility alleles in the tetraploid sour cherry. Theor Appl Genet 112, 315–326 (2006). https://doi.org/10.1007/s00122-005-0130-0

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  • DOI: https://doi.org/10.1007/s00122-005-0130-0

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