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Optimization of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material

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Abstract

Synthesis of amylase by Aspergillus niger strain UO-01 under solid-state fermentation with sugarcane bagasse was optimized by using response surface methodology and empirical modelling. The process parameters tested were particle size of sugarcane bagasse, incubation temperature and pH, moisture level of solid support material and the concentrations of inoculum, total sugars, nitrogen and phosphorous. The optimum conditions for high amylase production (457.82 EU/g of dry support) were particle size of bagasse in the range of 6–8 mm, incubation temperature and pH: 30.2°C and 6.0, moisture content of bagasse: 75.3%, inoculum concentration: 1 × 107 spores/g of dry support and concentrations of starch, yeast extract and KH2PO4: 70.5, 11.59 and 9.83 mg/g of dry support, respectively. After optimization, enzyme production was assayed at the optimized conditions. The results obtained corroborate the effectiveness and reliability of the empirical models obtained.

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Abbreviations

RSM:

Response surface methodology

SLF:

Submerged liquid fermentation

SSF:

Solid-state fermentation

gds:

g of dry support

TAA:

Total amylase activity

EU:

Enzymatic units

T:

Temperature

IC:

Inoculum concentration

M:

Moisture content

TS:

Total sugars concentration

N:

Nitrogen concentration

P:

Phosphorous concentration

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Authors and Affiliations

  1. Department of Pharmacy, Natural Science Faculty, University of Oriente, Santiago de Cuba, Cuba

    Renato Pérez Rosés

  2. Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, Ourense Campus, University of Vigo, As Lagoas, 32004, Ourense, Spain

    Nelson Pérez Guerra

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  1. Renato Pérez Rosés
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  2. Nelson Pérez Guerra
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Correspondence to Nelson Pérez Guerra.

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Rosés, R.P., Guerra, N.P. Optimization of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material. World J Microbiol Biotechnol 25, 1929–1939 (2009). https://doi.org/10.1007/s11274-009-0091-6

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  • DOI: https://doi.org/10.1007/s11274-009-0091-6

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