Summary
A number of nutritional factors affecting α-amylase production by the edible fungus Calvatia gigantea, cultivated in a wheat bran liquid medium, were investigated. The concentration and particle size of wheat bran and the relative concentration of three nitrogen and three mineral sources markedly affected enzyme production. A high amylase yield of 60.4 U ml-1 was obtained when the fungus was cultivated for 5 days at 29°C in a medium containing optimum concentrations of the tested factors. The results provide additional supporting evidence for the versatility of Calvatia gigantea to upgrade a number of low-cost agricultural products to high value biotechnological products.
Similar content being viewed by others
References
Alexopoulos CJ (1962) Introductory mycology. J Wiley & Sons Inc, New York
Aunstrup K (1979) Production, isolation and economics of extracellular enzymes. Appl Biochem Bioeng 2:27–69
Bernfeld P (1959) Amylases α and β. Methods Enzymol 1:49–158
Davies R (1964) Lactose utilization and hydrolysis in Saccharomyces fragilis. J Gen Microbiol 37:81–89
Fogarty WM (1983) Microbial enzymes and biotechnology. Applied Science Publishers, London
Galiotou-Panayotou M, Macris BJ (1980) On the mechanism of condensed tannin degradation by microorganisms. Evidence for a new enzyme breaking down catechin. VI International Fermentation Symposium and V International Symposium on Yeasts, London, Ontario, Canada, 20–25 July, Abstracts p 138
Galiotou-Panayotou M, Macris BJ (1986) Degradation of condensed tannins by Calvatia gigantea. Appl Microbiol Biotechnol 23:502–506
Glymph J, Stutzenberger F (1977) Production, purification and characterization of α-amylase from Thermomonospora curvata. Appl Environ Microbiol 34:391–397
Kassim EA (1983) Effect of nutrition and physiological conditions on the production of α-amylase and glucoamylase by a selected strain of Aspergillus oryzae. Mikrobiologiya 52:422–427
Kekos D, Macris BJ (1983a) Production of microbial protein from the fungus Calvatia gigantea grown on carbon sources containing high amounts of tannins. VI World Congress of Food Science and Technology, Dublin, Ireland, 18–23 Sept. Proceedings 2:165–166
Kekos D, Macris BJ (1983b) Production and characterization of amylase from Calvatia gigantea. Appl Environ Microbiol 45:935–941
Kekos D, Macris BJ (1986) Effect of tannins on growth and amylase production by Calvatia gigantea. Enzyme Microb Technol 9:94–96
Kundu AK, Das S (1970) Production of amylase in liquid culture by a strain of Aspergillus oryzae. Appl Microbiol 19:598–603
Macris B (1981) Production of extracellular lactase from Fusarium moniliforme. Eur J Appl Microbiol Biotechnol 13:161–164
Rao-Ramana MV, Dutta SM (1977) Production of beta-galactosidase from Streptococcus thermophilus grown in whey. Appl Environ Microbiol 34:185–188
Reed G (1966) Enzymes in food processing. Academic Press, New York
Rose AH, Harisson JC (1969) The yeasts, vol. 1. Academic Press, New York
Shannon LJ, Stevenson KE (1975) Growth of Calvatia gigantea and Candida steatolytica in brewery wastes for microbial protein production and BOD reduction. J Food Sci 40:830–832
Welker N, Campbell L (1963) Induced biosynthesis of α-amylase by growing cultures of Bacillus stearothermophilus. J Bacteriol 86:1197–1209
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kekos, D., Galiotou-Panayotou, M. & Macris, B.J. Some nutritional factors affecting α-amylase production by Calvatia gigantea . Appl Microbiol Biotechnol 26, 527–530 (1987). https://doi.org/10.1007/BF00253026
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00253026