Skip to main content
SpringerLink
Log in

Identification of three wheat near isogenic lines originated from CB037 on tissue culture and transformation capacities

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

To meet the increasing demand for wheat grains, it is essential to continuously improve wheat by commercial and advanced technologies. Wheat line CB037 has ideal agronomic traits, good bread quality, resistance to powdery mildew (PM), and high regeneration ability, and therefore it has been widely used in hybridization breeding and genetic transformation. Three near isogenic lines (NILs) from CB037 were identified in a previous study based on glutenin composition. In this study, the three NILs of CB037A, CB037B, and CB037C were investigated comparatively for agronomic traits, disease resistance to PM and stripe rust, and tissue culture and transformation efficiency. Results indicated that these NILs were highly resistant to PM, and CB037B was not only highly resistant to stripe rust but also had significantly higher regeneration ability than CB037A and CB037C in anther culture, immature and mature embryo culture. Particularly, positively transgenic wheat plants were obtained only from CB037B. Thereby CB037B carrying two types of translocations of 6AL·6V#2S and 1BL·1RS was identified to be a desirable material for wheat genetic transformation, which was of great significance for improving the transformation efficiency and accelerating the application of genetic engineering breeding technology in wheat.

Key message

CB037B was high resistant to PM and stripe rust. It had high regeneration ability in anther, immature, and mature embryo culture, and could be used in wheat genetic transformation for generating transgenic plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data availability

There are supplementary data in this manuscript and all data are included in the main text. The materials used in this study will be available upon request.

Abbreviations

2,4-D:

2,4-Dicholrophenoxyacetic acid

Bgt :

Blumeria graminis f. sp. tritici

CDR:

callus differentiated rate

CS:

Chinese Spring

DPA:

days post anthesis

ECIR:

embryogenic callus induction rate

GBTS:

genotyping by targeted sequencing

GISH:

genomic in situ hybridization

GUS:

ß-glucuronidase

InDels:

insertions and deletions (InDels)

KT:

kinetin

MCS:

multiple cloning site

NILs:

near isogenic lines

NOS:

nopaline synthase

PCR:

polymerase chain reaction

PM:

powdery mildew

PPT:

phosphinothricin

Pst :

Puccinia striiformis f. sp. tritici

SIR:

shoot induction rate

SNPs:

single nucleotide polymorphisms

ubi:

ubiquitin

References

  • Abebe T, Guenzi AC, Martin B, Cushman JC (2003) Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity. Plant Physiol 131:1748–1755

    Article  CAS  Google Scholar 

  • Bie XM, Dong L, Li XH, Wang H, Gao XQ, Li XG (2020) Trichostatin A and sodium butyrate promotes plant regeneration in common wheat. Plant Signal Behav 15:1820681

    Article  Google Scholar 

  • Bie XM, Wang K, Liu C, Liu YW, Du LP, Mao XG, Ye XG (2017) Effects of soil drought stress on plant regeneration efficiency and endogenous hormone levels of immature embryos in wheat (& Triticum aestivum L.). Pak J Bot 49:1673–1679

    CAS  Google Scholar 

  • Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol 115:971–980

    Article  CAS  Google Scholar 

  • Chen LF, Yan YP, Wang XD, Liu Y, Zhang HY, Zhang ZY (2013) Development and detection of wheat breeding materials resistant to both powdery mildew and barley yellow dwarf virus. J Plant Genetic Resour 14:925–929 (in Chinese with English abstract)

    Google Scholar 

  • Chen ZY, Ke WS, He F, Chai LL, Cheng XJ, Xu HW, Wang XB, Du DJ, Zhao YD, Chen XY, Xing JW, Xin MM, Guo WL, Hu ZR, Su ZQ, Liu J, Peng HR, Yao YY, Sun QX, Ni ZF (2022) A single nucleotide deletion in the third exon of FT-D1 increases the spikelet number and delays heading date in wheat (Triticum aestivum L.). Plant Biotechnol J 20:920–933

    Article  CAS  Google Scholar 

  • Dong LW, Li N, Lu XB, Prodanovic S, Xu YH, Zhang WY, Yan YM (2016) Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. Genetika 48:249–269

    Article  Google Scholar 

  • Guo X, Su HD, Shi QH, Fu SL, Wang J, Zhang XQ, Hu ZM, Han FP (2016) De novo centromere formation and centromeric sequence expansion in wheat and its wide hybrids. PLoS Genet 12:e1005997

    Article  Google Scholar 

  • Han DJ, Kang ZS (2008) Current status and future strategy in breeding wheat for resistance to stripe rust in China. Plant Prot 44:1–12 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Han J, Lakshman DK, Galvez LC, Mitra S, Baenziger PS, Mitra A (2012) Transgenic expression of lactoferrin imparts enhanced resistance to head blight of wheat caused by Fusarium graminearum. BMC Plant Biol 12:33–41

    Article  CAS  Google Scholar 

  • Hayta S, Smedley MA, Demir SU, Blundell R, Hinchliffe A, Atkinson N, Harwood WA (2019) An efficient and reproducible Agrobacterium mediated transformation method for hexaploid wheat (Triticum aestivum L.). Plant Methods 15:121

    Article  CAS  Google Scholar 

  • He ZH, Xia XC, Chen XM, Zhuang QS (2011) Progress of wheat breeding in China and the future perspective. Acta Agron Sinica 37:202–215 (in Chinese with English abstract)

    Article  Google Scholar 

  • Hu T, Metz S, Chay C, Zhou HP, Biest N, Chen G, Chen M, Feng X, Radionenko M, Lu F, Fry J (2003) Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection. Plant Cell Rep 21:1010–1019

    Article  CAS  Google Scholar 

  • Ishida Y, Tsunashima M, Hiei Y, Komari Y (2015) Wheat (Triticum aestivum L.) transformation using immature embryos. In: Wang K (ed) Agrobacterium Protocols: Volume 1. Methods in Molecular Biology, vol 1223. Springer Science + Business Media, New York, pp 189–198

    Chapter  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: betaglucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    Article  CAS  Google Scholar 

  • Khurana J, Chugh A, khurana P (2002) Regeneration from mature and immature embryos and transient gene expression via Agrobacteriummediated transformation in emmer wheat (Triticum diccocum Schuble). Indian J Exp Biol 40:1295–1303

    CAS  Google Scholar 

  • Li SJ, Jia ZM, Wang K, Du LP, Li HJ, Lin ZS, Ye XG (2020) Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033. Theor Appl Genet 133:3067–3083

    Article  CAS  Google Scholar 

  • Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968-87. U.S. Department of Agriculture, National Technical Information Service, Springfield, VA, USA. Technical Bulletin No. 1788

    Google Scholar 

  • Liu C, Li SJ, Wang K, Ye XG, Lin ZS (2017) Developing of Specific Transcription Sequences P21461 and P33259 on Dasypyrum villosum 6VS and Application of Molecular Markers in Identifying Wheat-D. villosum Breeding Materials with Powdery Mildew Resistance. Acta Agron Sinica 43:983–992 (in Chinese with English abstract)

    Article  Google Scholar 

  • Liu CX, Zhong Y, Qi XL, Chen M, Liu ZK, Chen C, Tian XL, Li JL, Jiao YY, Wang D, Wang YW, Li MR, Xin MM, Liu WX, Jin WW, Chen SJ (2020a) Extension of the in vivo haploid induction system from diploid maize to hexaploid wheat. Plant Biotechnol J 18:316–318

    Article  Google Scholar 

  • Liu HY, Wang K, Jia ZM, Gong Q, Lin ZS, Du LP, Pei XW, Ye XG (2020b) Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPR system. J Exp Bot 71:1337–1349

    Article  CAS  Google Scholar 

  • Mendoza MG, Kaeppler HF (2002) Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat (Triticum aestivum L.). Vitro Cell Dev Biology Plant 38:39–45

    Article  CAS  Google Scholar 

  • Ogawa T, Kawahigashi H, Toki S, Handa H (2008) Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker. Plant Cell Rep 27:1325–1331

    Article  CAS  Google Scholar 

  • Orshinsky BR, Sadasivaiah RS (1997) Effect of plant growth conditions, plating density, and genotype on the anther culture response of soft white spring wheat hybrids. Plant Cell Rep 16:758–762

    Article  CAS  Google Scholar 

  • Patnaik D, Khurana P (2003) Genetic transformation of Indian bread (T. aestivum) and pasta (T. durum) wheat by particle bombardment of mature embryo-derived calli. BMC Plant Biol 3:5–15

    Article  Google Scholar 

  • Pellegrineschi A, Noguera LM, Skovmand B, Brito RM, Velazquez L, Salgado MM, Hernandez R, Warburton M, Hoisington D (2002) Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants. Genome 45:421–430

    Article  CAS  Google Scholar 

  • Richardson T, Thistleton J, Higgins TJ, Howitt C, Ayliffe M (2014) Efficient Agrobacterium transformation of elite wheat germplasm without selection. Planr Cell Tissue Organ Cult 119:647–659

    Article  CAS  Google Scholar 

  • Shah M, Khalid Q, Khan U, Shah S, Shah S, Hassan A, Pervez A, Oliveira V, Caxito F, Gomes K (2009) Variation in genotypic responses and biochemical analysis of callus induction in cultivated wheat. Genet Mol Res 8:783–793

    Article  CAS  Google Scholar 

  • Sharma VK, Hnsch R, Mendel RR, Schulze J (2005) Influence of picloram and thidiazuron on high frequency plant regeneration in elite cultivars of wheat with long-term retention of morphogenecity using meristematic shoot segments. Plant Breeding 124:242–246

    Article  CAS  Google Scholar 

  • She MY, Yin GX, Li JR, Li X, Du LP, Ma WJ, Ye XG (2013) Efficient Regeneration potential is closely related to auxin exposure time and catalase metabolism during the somatic embryogenesis of immature embryos in Triticum aestivum L. Mol Biotechnol 54:451–460

    Article  CAS  Google Scholar 

  • Tao LL, Yin GX, Du LP, Shi ZY, She MY, Xu HJ, Ye XG (2011) Improvement of plant regeneration from immature embryos of wheat Infected by Agrobacterium tumefaciens. Agricultural Sci China 10:317–326

    Article  CAS  Google Scholar 

  • Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327:818–822

    Article  CAS  Google Scholar 

  • Wang C, Zeng J, Li Y, Hu W, Chen L, Miao YJ, Deng PY, Yuan CH, Ma C, Chen X, Zang ML, Wang Q, Li KX, Chang JL, Wang YS, Yang GX, He GY (2014a) Enrichment of provitamin A content in wheat (Triticum aestivum L.) by introduction of the bacterial carotenoid biosynthetic genes CrtB and CrtI. J Exp Bot 65:2545–2556

    Article  CAS  Google Scholar 

  • Wang K, Liu HY, Du LP, Ye XG (2017) Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties. Plant Biotechnol J 15:614–623

    Article  CAS  Google Scholar 

  • Wang XM, Ren X, Yin GX, Wang K, Li JR, Du LP, Xu HJ, Ye XG (2014b) Effects of environmental temperature on the regeneration frequency of the immature embryos of wheat (Triticum aestivum L.). J Integr Agric 13:722–732

    Article  Google Scholar 

  • Wang YP, Zhen SM, Luo NN, Han CX, Lu XB, Li XH, Xia XC, He ZH, Yan YM (2016) Low molecular weight glutenin subunit gene Glu-B3h confers superior dough strength and breadmaking quality in wheat (Triticum aestivum L.). Sci Rep 6:27182

    Article  CAS  Google Scholar 

  • Wu H, Doherty A, Jones HD (2008) Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Res 17:425–436

    Article  CAS  Google Scholar 

  • Wu JS, Lu XB, Yu ZT, Han CX, Li XH, Prodanovic S, Yan YM (2017) Effects of Glu-1 and Glu-3 allelic variation on wheat glutenic macropolymer content as revealed by size-exclusion high performance liquid chromatography (SE-HPLC). Genetika 49: 677–691

  • Xiao MG, Song FJ, Jiao JF, Wang XM, Xu HX, Li HJ (2013) Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet 126:1397–1403

    Article  CAS  Google Scholar 

  • Ye XG, Kang L, Xu HJ, Du LP (2005) Transferring glucose oxidase gene into wheat by biolistic particle. Acta Agron Sinica 31:686–691 (in Chinese with English abstract)

    Google Scholar 

  • Ye X, Xu HJ, Du LP, He GY, Wang K, Lin ZS (2014) Establishment and application of large-scale transformation systems in wheat. Scientia Agricultura Sinica 47:4155–4171 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Ye XG, Xu HJ, Xu QF, Du LP, Li ZW (1997) Genetic analysis and combining ability evaluation of the anther culture response in common wheat. Scientia Agricultura Sinica 30:49–54 (in Chinese with English abstract)

    Google Scholar 

  • Ye XG, Xu HJ, Zhao LL, Du LP (1998) Studies on improving wheat cultivars by tissue culture. Acta Agron Sinica 24:310–314 (in Chinese with English abstract)

    Google Scholar 

  • Yin GX, Wang YL, She MY, Du LP, Xu HJ, Ma JX, Ye XG (2011) Establishment of a highly efficient regeneration system for the mature embryo culture of wheat. Agricultural Sci China 10:9–17

    Article  CAS  Google Scholar 

  • Yuan J, Guo X, Hu J, Lv ZL, Han FP (2014) Characterization of two CENH3 genes and their roles in wheat evolution. New Phytol 206:839–851

    Article  Google Scholar 

  • Zhang L, Rybczynski JJ, Langenberg WG, Mitra A, French R (2000) An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration. Plant Cell Rep 19:241–250

    Article  CAS  Google Scholar 

  • Zhang W, Yin MQ, Zhao P, Wang K, Du LP, Ye XG (2018) Regeneration capacity evaluation of some largely popularized wheat varieties in China. Acta Agron Sinica 44:208–217 (in Chinese with English abstract)

    Article  Google Scholar 

  • Zhang YL, Wand MJ, Zhang Y, Chu CP, Lin ZS, Xu QF, Ye XG, Chen X, Zhang XS (2012) Development and application of functional markers specific to powdery mildew resistance on chromosome arm 6VS from different origins of Haynaldia villosa. Acta Agron Sinica 38:1827–1832 (in Chinese with English abstract)

    Article  CAS  Google Scholar 

  • Zhao LS, Liu LX, Wang J, Guo HJ, Gu JY, Zhao SR, Li JH, Xie YD (2015) Development of a new wheat germplasm with high anther culture ability by using a combination of gamma-ray irradiation and anther culture. J Sci Food Agric 95:120–125

    Article  CAS  Google Scholar 

  • Zheng YY, Huang DH, Li JL, Zhang HF, Bao YG, Ni F, Wu JJ (2020) Analysis of the stripe rust resistance in a wheat line CB037 with high regeneration and transformation efficiency. Acta Agron Sinica 46:1743–1749 (in Chinese with English abstract)

    Google Scholar 

  • Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylor ML, Rodriguez D, Pajeau ME, Brown SM, Santino CG, Fry JE (1995) Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Rep 15:159–163

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank Dr. Fangpu Han, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for his guidance on genomic in situ hybridization of wheat chromosomes. This study was financially supported by the National Natural Science Foundation of China (31971945) and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (2060299-2-22).

Author information

Author notes

    Authors and Affiliations

    1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

      Huiyun Liu, Haili Ma, Wei Zhang, Wanqing Wang, Ke Wang, Zhishan Lin & Xingguo Ye

    2. College of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, 450002, Zhengzhou, Henan, China

      Huiyun Liu

    3. Gansu Dunhuang Forestry Technology Extension and Service Center, 736200, Dunhuang, Gansu, China

      Haili Ma

    4. State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, 271018, Tai’an, Shandong, China

      Jiajie Wu

    Authors
    1. Huiyun Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Haili Ma
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Wei Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Wanqing Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Jiajie Wu
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Ke Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Zhishan Lin
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Xingguo Ye
      View author publications

      You can also search for this author in PubMed Google Scholar

    Contributions

    XY and ZL conceived the research and designed the experiments. HL conducted most of the experiments. HM and ZL performed cytogenetics observation. WZ and WW participated in tissue culture. HM, ZL and JW identified PM and stripe rust resistance. KW performed vector construction and participated in genetic transformation. HL and XY analyzed the data, and drafted and revised the manuscript.

    Corresponding authors

    Correspondence to Zhishan Lin or Xingguo Ye.

    Ethics declarations

    Conflict of interest

    The authors declare that they have no conflict of interest.

    Ethics approval

    Not applicable.

    Consent to participate

    Not applicable.

    Consent for publication

    Not applicable.

    Additional information

    Communicated by Shabir Hussain Wani.

    Publisher’s note

    Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

    Huiyun Liu and Haili Ma contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary Material 1

    Rights and permissions

    Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Liu, H., Ma, H., Zhang, W. et al. Identification of three wheat near isogenic lines originated from CB037 on tissue culture and transformation capacities. Plant Cell Tiss Organ Cult 152, 67–79 (2023). https://doi.org/10.1007/s11240-022-02389-z

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s11240-022-02389-z

    Keywords

    Search

    Navigation