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Introgression of recombinant 1RSWW.1BL without secalin and with Glu-B3/Gli-B1 into elite wheat cultivars through marker-assisted backcross breeding

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Abstract

The wheat–rye 1RS.1BL translocation has been extensively utilized in wheat breeding programs across the world for multiple disease resistances, abiotic stress tolerance and higher grain yield. The Sec-1 locus present on the 1RS and codes for rye storage protein secalin which causes stickiness in the dough leading to poor bread quality. In addition to this, the absence of Glu-B3/Gli-B1 on 1BS in this translocation further deteriorates the bread-making quality of wheat. In the present study, Pavon MA1 was used as the donor parent, and two elite wheat cultivars HS240 and UP2338, having 1RS.1BL translocation, were used as the recipient parents. Pavon MA1 has 1RSWW.1BL translocation in which the Sec-1 locus is absent whereas Glu-B3/Gli-B1 is present. This 1RSWW.1BL translocation was introgressed in the two wheat cultivars using marker-assisted backcross breeding, and BC2F5 derivatives were developed for both the cultivars. The dough quality of these derivatives was analyzed through the micro-SDS-sedimentation test (MST), high-molecular-weight glutenin subunits (HMWGS), loaf volume and micro-dough laboratory test. The dough analysis showed that the elimination of secalin in the derivatives led to the higher loaf volume, dough development time, dough stability and water absorption thus improving its bread-making quality.

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Acknowledgements

The authors would like to acknowledge Dr. Khem Singh Gill, Akal College of Agriculture, Eternal University for providing the necessary infrastructure and research facilities for carrying out this research work.

Funding

This project was financially supported by the Department of Biotechnology, Government of India (BT/ PR10886/AGII/106/934/2014).

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Authors and Affiliations

  1. Department of Genetics-Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, HP, 173101, India

    Ambika Sharma, Pritesh Vyas, H. S. Dhaliwal & Imran Sheikh

  2. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India

    Satinder Kaur

  3. Food and Nutritional Biotechnology, National Agri-Food Biotechnology Institute, Mohali, Punjab, 140306, India

    Monika Garg

  4. Department of Food Technology, Guru Jambheshwar University, Hisar, Haryana, 125001, India

    Rajesh Kumar & B. S. Khatkar

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  1. Ambika Sharma
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  2. Satinder Kaur
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  3. Monika Garg
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  4. Rajesh Kumar
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  5. B. S. Khatkar
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  6. Pritesh Vyas
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  7. H. S. Dhaliwal
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  8. Imran Sheikh
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Correspondence to H. S. Dhaliwal or Imran Sheikh.

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Communicated by Márta Molnár-Láng.

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Sharma, A., Kaur, S., Garg, M. et al. Introgression of recombinant 1RSWW.1BL without secalin and with Glu-B3/Gli-B1 into elite wheat cultivars through marker-assisted backcross breeding. CEREAL RESEARCH COMMUNICATIONS 52, 867–874 (2024). https://doi.org/10.1007/s42976-023-00419-w

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  • DOI: https://doi.org/10.1007/s42976-023-00419-w

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