Abstract
Wheat may now be transformed very efficiently by Agrobacterium tumefaciens. Under the protocol hereby described, immature embryos of healthy plants of wheat cultivar Fielder grown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with A. tumefaciens. Transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. All regenerants were normal in morphology and fully fertile. About half of the transformed plants carried single copy of the transgene, which are inherited by the progeny in a Mendelian fashion.
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Acknowledgments
We thank Dr. Elizabeth E. Hood for the gift of EHA101, Dr. Stanton B. Gelvin for the gift of EHA105, Dr. Kenzo Nakamura for the gift of pIG121Hm, and Ms. Eriko Usami for technical assistance.
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Ishida, Y., Tsunashima, M., Hiei, Y., Komari, T. (2015). Wheat (Triticum aestivum L.) Transformation Using Immature Embryos. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_15
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DOI: https://doi.org/10.1007/978-1-4939-1695-5_15
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