Abstract
A method for producing large numbers of transgenic wheat plants has been developed. With this approach, an average of 9.7% of immature embryo explants were transformed and generated multiple self-fertile, independently transformed plants. No untransformed plants, or escapes, were regenerated. This transformation procedure uses morphogenic calli derived from scutellum tissue of immature embryos of Triticum aestivum cv. Bobwhite co-bombarded with separate plasmids carrying a selectable marker gene (bar) and a gene of interest, respectively. Transformed wheat calli with a vigorous growth phenotype were obtained by extended culture on media containing 5.0 mg/l bialaphos. These calli retained morphogenic potential and were competent for plant regeneration for as long as 11 months. The bar gene and the gene of interest were co-expressed in T0 progeny plants. This wheat transformation protocol may facilitate quantitative production of multiple transgenic plants and significantly reduce the cost and labor otherwise required for screening out untransformed escapes.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 15 June 1998 / Revision received: 6 April 1999 / Accepted: 26 April 1999
Rights and permissions
About this article
Cite this article
Zhang, L., Rybczynski, J., Langenberg, W. et al. An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration. Plant Cell Reports 19, 241–250 (2000). https://doi.org/10.1007/s002990050006
Issue Date:
DOI: https://doi.org/10.1007/s002990050006