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Effect of macronutrients and micronutrients on biochemical properties in Paulownia shantung

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Abstract

Paulownia (Paulownia sp) is a fast-growing, industrial and ornamental tree, which has medicinal usages. The effect of nutrients in in vitro regenerated plantlets and somaclonal variation was investigated. Biochemical traits (soluble leaf protein, anthocyanin, flavonoid, proline, carotenoid contents and photosynthetic pigment amounts) in induced embryos and regenerated plantlets were measured. The media of A/2 MS, half-strength MS, and ½&A/4 MS supplemented with a combination of TDZ, 2,4-D, Kin, BAP and GA3 hormones -eight media totally- were used. The shortest duration for direct and indirect somatic embryogenesis was observed in media number eight (½&A/4 MS + 0.3 mg/l 2,4-D + 2 mg/l Kin + 4 mg/l BAP + 0.3 mg/l GA3). Moreover, this medium showed the highest frequency in calli induction, somatic embryos and regenerations. We showed the privilege of full-strength MS in an in vitro propagation of paulownia with no-somaclonal and genomic alterations. For the media with reduced nutrient strength to one-fourth and one-second, somaclonal variation and genomic changes were recorded. Media with decreased macronutrients to half and a quarter amount showed a higher ratio of somatic embryo induction, regeneration, and antioxidant activity. Meanwhile, proline and protein contents were decreased. An increase in chlorophyll content was observed in media with reduced macronutrients amount. Overall, elements may be linked to the frequency of somaclonal variation in an in vitro culture.

Key message

By reducing macronutrients, especially nitrate and ammonium in tissue culture of paulownia plant, somatic embryo induction, regeneration and antioxidant activities increase while, free radicals reduce.

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Data Availability

The authors declare that the data supporting the findings of this study are available within the article.

Abbreviations

A:

Absorbance

BAP:

6-Benzylaminopurine

GA3 :

Gibberellic acid

MS:

Murashige and skoog

A/2 MS:

Reduction to half of macronutrients in the full-strength MS

½ MS:

Reduction to half the entire full-strength MS content

½&A/4MS:

Reduction to half than twice the entire contents of the medium and reduction to a quarter of the macronutrients in the full-strength MS

2,4-D:

2,4-Dichlorophenoxyacetic acid

Kin:

Kinetin

V:

Volume

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Acknowledgements

We thank and appreciate the University of Tehran science & technology park for financial support.

Funding

Funding was provided by University of Tehran Science & Technology Park (Grant No. 120021).

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, PO Box 3391653755, Tehran, Iran

    Yasin Dumani, Seyed Mohammad Mahdi Mortazavian, Ali Izadi-Darbandi & Hossein Ramshini

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  1. Yasin Dumani
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  2. Seyed Mohammad Mahdi Mortazavian
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  3. Ali Izadi-Darbandi
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Correspondence to Seyed Mohammad Mahdi Mortazavian.

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Communicated by Ewa Grzebelus.

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Dumani, Y., Mortazavian, S.M.M., Izadi-Darbandi, A. et al. Effect of macronutrients and micronutrients on biochemical properties in Paulownia shantung. Plant Cell Tiss Organ Cult 151, 483–495 (2022). https://doi.org/10.1007/s11240-022-02365-7

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