Skip to main content
SpringerLink
Log in

Differential antioxidative responses of indica rice cultivars to drought stress

  • Original Paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The present study investigated the linkages between drought stress, oxidative damages and variations in antioxidants in the three rice varieties IR-29 (salt-sensitive), Pokkali (salt-tolerant) and aromatic Pusa Basmati (PB), to elucidate the antioxidative protective mechanism governing differential drought tolerance. Water deficit, induced by 20% (w/v) polyethylene glycol (PEG-6000), provoked severe damages in IR-29 and PB in the form of huge chlorophyll degradation and elevated H2O2, malondialdehyde and lipoxygenase (LOX, EC 1.13.11.12) levels as compared to Pokkali. The protein oxidation was more conspicuous in IR-29. Increment in antioxidants, particularly flavonoids and phenolics was several folds higher over control in Pokkali, while much lesser in IR-29 and PB. The activity of catalase (CAT, EC 1.11.1.6) and superoxide dismutase (SOD, EC 1.15.1.1) were decreased in IR-29 and PB, but unaltered in Pokkali. However, marked drought-induced increase in guaiacol peroxidase (GPX, EC 1.11.1.7) activity was noted in both IR-29 and PB. Induction in radical scavenging activity, being the maximum in IR-29, and increased reducing power ability in all the cultivars, accompanied with drought stress, were observed as a defense mechanism. The novelty of our work is that it showed the aromatic rice PB behaving more closely to IR-29 in greater susceptibility to dehydration stress, while the salt-tolerant Pokkali also showed effective drought tolerance properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

BSA:

Bovine serum albumin

CAT:

Catalase

DNP:

Dinitrophenyl

DPPH:

1, 1-Diphenyl-2-picrylhydrazyl

GPX:

Guaiacol peroxidase

LOX:

Lipoxygenase

MDA:

Malondialdehyde

PEG:

Polyethylene glycol

SDS:

Sodium dodecyl sulfate

SOD:

Superoxide dismutase

References

  • Alonso R, Elvira S, Castillo FJ, Gimeno BS (2001) Interactive effects of ozone and drought stress on pigments and activities of antioxidative enzymes in Pinus halepensis. Plant Cell Environ 24:905–916

    Article  CAS  Google Scholar 

  • Angelopoulos K, Dichio B, Xiloyannis C (1996) Inhibition of photosynthesis in olive trees (Olea europaea L.) during water stress and rewatering. J Exp Bot 47:1093–1100

    Article  CAS  Google Scholar 

  • Bandurska H (2000) Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants. Acta Physiol Plant 22:409–415

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  PubMed  Google Scholar 

  • Benard FJ, Runner RTM (2004) Erythrinaline alkaloids from the flowers and pods of Erythrina lysistemon and their DPPH radical scavenging properties. Phytochemistry 65:1397–1401

    Article  Google Scholar 

  • Berlett BS, Stadtman ER (1997) Protein oxidation in aging, disease and oxidative stress. J Biol Chem 272:20313–20316

    Article  CAS  PubMed  Google Scholar 

  • Bhargava S, Paranjpe S (2004) Genotyping variation in the photosynthetic competence of Sorghum bicolor seedlings subjected to polyethylene glycol-mediated drought stress. J Plant Physiol 161:125–129

    Article  CAS  PubMed  Google Scholar 

  • Chalker-Scott L (1999) Environmental significance of anthocyanins in plant stress response. Photochem Photobiol 70:1–9

    Article  CAS  Google Scholar 

  • Chang-Quan W, Rui-Chang L (2008) Enhancement of superoxide dismutase activity in the leaves of white-clover (Trifolim repens L.) in response to polyethylene glycol-induced water stress. Acta Physiol Plant 30:841–847

    Article  Google Scholar 

  • Couper A, Eley D (1984) Surface tension of polyethylene glycol solutions. J Polym Sci 3:345–349

    Article  Google Scholar 

  • Dat JF, Lopez-Delgado H, Foyer CH, Scott IM (1998) Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiol 116:1351–1357

    Article  CAS  PubMed  Google Scholar 

  • Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9

    Article  CAS  Google Scholar 

  • Duh P-D (1998) Antioxidant activity of Budrock (Arctium laooa Linn): its scavenging effect on free radical and active oxygen. J Am Oil Chem Soc 75:455–461

    Article  CAS  Google Scholar 

  • Dwivedi S, Kar M, Misra D (1979) Biochemical changes in excised leaves of Oryza sativa subjected to water stress. Physiol Plant 45:34–40

    Article  Google Scholar 

  • Gao S, Luo J, Zhang H, Chen R, Lin Y (2006) Physiological and biochemical indexes of drought resistance of sugarcane (Saccharum spp.). Ying Yong Sheng Tai Xue Bao 17:1051–1054

    CAS  PubMed  Google Scholar 

  • Hernandez I, Alegre L, Munne-Bosch S (2004) Drought-induced changes in flavonoids and other low molecular-weight antioxidants in Cistus clusii grown under Mediterranean field conditions. Tree Physiol 24:1303–1311

    CAS  PubMed  Google Scholar 

  • Horváth E, Pál M, Szalai G, Páldi E, Janda T (2007) Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants. Biol Plant 51:480–487

    Article  Google Scholar 

  • Ithal N, Reddy AR (2004) Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Sci 166:1505–1513

    Article  CAS  Google Scholar 

  • Jagtap V, Bhargava S (1995) Variations in the antioxidant metabolism of drought-tolerant and drought-susceptible varieties of Sorghum bicolor (L.) Moench. exposed to high light, low water and high temperature stress. J Plant Physiol 145:195–197

    CAS  Google Scholar 

  • Jayaprakasha GK, Singh RP, Sakariah KK (2001) Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem 73:285–290

    Article  CAS  Google Scholar 

  • Jia Z, Tang M, Fu J (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

    Article  Google Scholar 

  • Jung S (2004) Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Plant Sci 166:459–466

    Article  CAS  Google Scholar 

  • Kang HM, Saltveit ME (2002a) Effect of chilling on antioxidant enzymes and DPPH-radical scavenging activity of high and low-vigour cucumber seedling radicles. Plant Cell Environ 25:1233–1238

    Article  CAS  Google Scholar 

  • Kang H-M, Saltveit ME (2002b) Antioxidant enzymes and DPPH-radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles. J Agric Food Chem 50:513–518

    Article  CAS  PubMed  Google Scholar 

  • Kumaran A, Karunakaran RJ (2006) Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 97:109–114

    Article  CAS  Google Scholar 

  • Lee DH, Kim YS, Lee CB (2001) The inductive responses of the antioxidant enzymes by salt stress in rice (Oryza sativa L.). J Plant Physiol 158:737–745

    Article  CAS  Google Scholar 

  • Lima ALS, DaMatta FM, Pinheiro HA, Totola MR, Loureiro ME (2002) Photochemical responses and oxidative stress in two clones of Coffea canephora under water deficit conditions. Environ Exp Bot 47:239–247

    Article  CAS  Google Scholar 

  • Menconi M, Sgherri CLM, Pinzino C, Navari-Izzo F (1995) Activated oxygen production and detoxification in wheat plants subjected to a water deficit programme. J Exp Bot 46:1123–1130

    Article  CAS  Google Scholar 

  • Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol-6000. Plant Physiol 51:914–916

    Article  CAS  PubMed  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  PubMed  Google Scholar 

  • Moran JF, Becana M, Iturbe-Ormaetxe I, Frechilla S, Klucas RV, Aparicio-Tejo P (1994) Drought induces oxidative stress in pea plants. Planta 194:346–352

    Article  CAS  Google Scholar 

  • Mukherjee SP, Choudhuri MA (1983) Implication of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170

    Article  CAS  Google Scholar 

  • Nathawat NS, Nair JS, Kumawat SM, Yadava NS, Singh G, Ramaswamy NK, Sahu MP, D’Souza SF (2007) Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress. Biol Plant 51:93–97

    Article  CAS  Google Scholar 

  • Navari-Izzo F, Quartacci MF, Izzo R (1990) Water stress induced changes in protein and free amino acids in field-grown maize and sunflower. Plant Physiol Biochem 28:531–537

    CAS  Google Scholar 

  • Nguyen TTT, Klueva N, Chamareck V, Aarti A, Magpantay G, Millena ACM, Pathan MS, Nguyen HT (2004) Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Genet Genomics 272:35–46

    Article  CAS  PubMed  Google Scholar 

  • Palma JM, Sandalio LM, Corpas FJ, Romero-Puertas MC, Mccarthy I, Delrio LA (2002) Plant proteases, protein degradation and oxidative stress: role of peroxisomes. Plant Physiol Biochem 40:521–530

    Article  CAS  Google Scholar 

  • Polle A (1997) Defense against photooxidative damage in plants. In: Scandalios J (ed) Oxidative stress and the molecular biology of antioxidant defenses. Cold Spring Harbor Laboratory Press, NY, pp 785–813

    Google Scholar 

  • Reddy AR, Chaitanya KV, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202

    Article  CAS  Google Scholar 

  • RoyChoudhury A, Roy C, Sengupta DN (2007) Transgenic tobacco plants over expressing the heterologous lea gene Rab16A from rice during high salt and water deficit display enhanced tolerance to salinity stress. Plant Cell Rep 26:1839–1859

    Article  CAS  PubMed  Google Scholar 

  • Roychoudhury A, Basu S, Sarkar SN, Sengupta DN (2008) Comparative physiological and molecular responses of a common aromatic indica rice cultivar to high salinity with non-aromatic indica rice cultivars. Plant Cell Rep 27:1395–1410

    Article  CAS  PubMed  Google Scholar 

  • Roychoudhury A, Basu S, Sengupta DN (2009) Effects of exogenous abscisic acid on some physiological responses in a popular aromatic indica rice compared with those from two traditional non-aromatic indica rice cultivars. Acta Physiol Plant 31:915–926

    Article  CAS  Google Scholar 

  • RoyChowdhury S, Choudhuri MA (1985) Hydrogen peroxide metabolism as an index of water stress tolerance in jute. Physiol Plant 65:503–507

    Article  Google Scholar 

  • RoyChowdhury S, Choudhuri MA (1989) Effect of CaCl2 and ABA on changes in H2O2 metabolism in two jute species under water deficit stress. J Plant Physiol 135:179–183

    Google Scholar 

  • Roy-Macauley R, Zuily-Fodii Y, Kidric M, Pham Thi AT, Vieira da Silva J (1992) Effect of drought stress on proteolytic activities in Phaseolus and Vigna leaves from sensitive and resistant plants. Physiol Plant 85:90–96

    Article  CAS  Google Scholar 

  • Sairam RK, Saxena DC (2000) Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. J Agron Crop Sci 184:55–61

    Article  CAS  Google Scholar 

  • Sairam RK, Deshmukh PS, Saxena DC (1998) Role of antioxidant systems in wheat genotypes tolerance to water stress. Biol Plant 41:387–394

    Article  CAS  Google Scholar 

  • Sgherri CLM, Maffei M, Navari-Izzo F (2000) Antioxidative enzymes in wheat subjected to increasing intensity of water deficit and rewatering. J Plant Physiol 157:273–279

    CAS  Google Scholar 

  • Sgherri C, Stevanovic B, Navari-Izzo F (2004) Role of phenolic acid during dehydration and rehydration of Ramonda serbica. Physiol Plant 122:478–485

    Article  CAS  Google Scholar 

  • Sinhababu A, Kar RK (2003) Comparative responses of three fuel wood yielding plants to PEG-induced water stress at seedling stage. Acta Physiol Plant 25:403–409

    Article  Google Scholar 

  • Smirnoff N (1993) The role of active oxygen in the response to water deficit and desiccation. New Phytol 125:27–58

    Article  CAS  Google Scholar 

  • Sofo A, Dichio B, Xiloyannis C, Masia A (2004) Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. Physiol Plant 121:58–65

    Article  CAS  PubMed  Google Scholar 

  • Strzalka K, Kostecka-Gugala A, Latowski D (2003) Carotenoids and environmental stress in plants: significance of carotenoid-mediated modulation of membrane physical properties. Russ J Plant Physiol 50:168–173

    Article  CAS  Google Scholar 

  • Sun CP, Zhang JZ, Duan SJ (1999) Free radical biology. Science and Technology University of China Press, Hefei

    Google Scholar 

  • Tsuda T, Shiga K, Ohshima K, Kawakishi S, Osawa T (1996) Inhibition of lipid peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L. Biochem Pharmacol 52:1033–1039

    Article  CAS  PubMed  Google Scholar 

  • Van Rensburg L, Kruger G (1994) Evaluation of components of oxidative stress metabolism for use in selection of drought tolerant cultivars of Nicotiana tabacum L. J Plant Physiol 145:730–737

    Google Scholar 

  • Verbeke P, Siboska GE, Clark BFC, Rattan SIS (2000) Kinetin inhibits protein oxidation and glycoxidation in vitro. Biochem Biophys Res Commun 276:1265–1270

    Article  CAS  PubMed  Google Scholar 

  • Verslues PE, Ober ES, Sharp RE (1998) Root growth and oxygen relations at low water potentials. Impact of oxygen availability in polyethylene glycol solutions. Plant Physiol 116:1403–1412

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Kirkham MB (1994) Drought stress induced change in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol 35:785–791

    CAS  Google Scholar 

Download references

Acknowledgments

Financial support from Bose Institute and Department of Biotechnology (DBT), Government of India, New Delhi, are gratefully acknowledged.

Author information

Author notes

  1. Aryadeep Roychoudhury

    Present address: Department of Botany, Plant Molecular Biology and Biotechnology Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal, 700 019, India

Authors and Affiliations

  1. Department of Botany, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata, West Bengal, 700 009, India

    Supratim Basu, Aryadeep Roychoudhury, Progya Paromita Saha & Dibyendu N. Sengupta

Authors
  1. Supratim Basu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aryadeep Roychoudhury
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Progya Paromita Saha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dibyendu N. Sengupta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dibyendu N. Sengupta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Basu, S., Roychoudhury, A., Saha, P.P. et al. Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regul 60, 51–59 (2010). https://doi.org/10.1007/s10725-009-9418-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-009-9418-4

Keywords

Search

Navigation