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Efficient production of vigorous passion fruit rootstock for in vitro grafting

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Abstract

In vitro grafting is one of the promising techniques for fruit crop breeding. This study was performed to produce in vitro vigorous passion fruit rootstocks and developed a simple in vitro grafting protocol with the support of nylon microtubes—a low cost, wide availability, non-toxic and reusable material. The results showed that stem-lTCL explants (longitudinal thin cell layers) cultured on MS medium containing 0.5 mg/L BA and 0.5 mg/L NAA gave the highest shoot regeneration, and these shoots were initial materials for micropropagation of passion fruit rootstock. MSM medium containing 5 mg/L AgNPs increased shoot multiplication twofold higher than that of control and significantly reduced leaf yellowing and leaf drop during this stage. Rooting rate and quality of the plantlets were optimal on MSM medium contained with 2.5 mg/L IBA as well as their survival rate was enhanced in nursery. Nylon microtubes improved the efficiency of passion fruit in vitro grafts to 73.33% with yellow passion fruit as rootstock and purple passion fruit nodal segments as scion. In addition, the use of nylon microtubes significantly improved the in vitro growth of in vitro grafted plants resulting in increased survival rate and plantlet growth in the nursery stage. The present results provide an efficient protocol for micropropagation of yellow passion fruit for rootstock and production of purple passion fruit via nylon microtubule-mediated in vitro grafting.

Key message

An efficient protocol was established for multiplication of in vitro vigorous passion fruit rootstock via the application of thin cell layer culture techniques, nanotechnology and modification of the culture medium. Nylon microtubes enhanced the success rate of in vitro grafting and ex vitro grafted plant growth.

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Acknowledgements

This work was supported by the National Foundation for Science and Technology Development (NAFOSTED), Vietnam under project code 106.01-2020.10.

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Authors and Affiliations

  1. Tay Nguyen Institute for Scientific Research, VAST, Dalat, Vietnam

    Tran Hieu, Truong Hoai Phong, Hoang Dac Khai, Nguyen Thi Nhu Mai, Do Manh Cuong, Vu Quoc Luan, Hoang Thanh Tung & Duong Tan Nhut

  2. University of Sciences, Hue University, Hue, Vietnam

    Tran Hieu

  3. Nong Lam University, Ninh Thuan Sub-Campus, Ho Chi Minh City, Vietnam

    Tran Hieu

  4. Graduate University of Science and Technology, VAST, Hanoi, Vietnam

    Truong Hoai Phong

  5. Dalat University, Da Lat, Vietnam

    Nguyen Ba Nam

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  1. Tran Hieu
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  2. Truong Hoai Phong
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  4. Nguyen Thi Nhu Mai
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  5. Do Manh Cuong
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  7. Hoang Thanh Tung
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  9. Duong Tan Nhut
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Contributions

NTD, Tran Hieu and Hoang Dac Khai designed the study. Truong Hoai Phong, Nguyen Thi Nhu Mai, Do Manh Cuong, Vu Quoc Luan, Hoang Thanh Tung and Nguyen Ba Nam performed the experiments and analyzed the data. Tran Hieu, Nhut Tan Duong and Hoang Dac Khai wrote the original draft.

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Correspondence to Duong Tan Nhut.

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Hieu, T., Phong, T.H., Khai, H.D. et al. Efficient production of vigorous passion fruit rootstock for in vitro grafting. Plant Cell Tiss Organ Cult 148, 635–648 (2022). https://doi.org/10.1007/s11240-021-02220-1

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