Abstract
This is the first report on isolated microspore culture—derived spelt wheat. The efficiency of anther- and isolated microspore was compared using four genotypes (‘Franckenkorn’, ‘GK Fehér’, ‘Mv Martongold’, ‘Oberkulmer Rotkorn’). In anther culture, genotype dependency was observed, and cold pre-treatment enhanced the efficiency of the method. In isolated microspore culture, the ovary co-culture supported the development of embryo-like structures. The presence of growth regulators (0.5 mg/l 2,4-D and 0.5 mg/l kinetin) were not essential for the induction of androgenesis, but these increased the production of embryo-like structures, green and albino plantlets. The low plant regeneration rate and high number of albinos hinder the practical application of isolated microspore culture while anther culture was efficient for in vitro green plantlets production in spelt wheat. The mean of green plantlets production was 41.45/100 anthers (from 20.93 to 83.07 depending on genotype). The phenomenon of albinism was mitigated in anther culture (3.48 albinos/100 anthers). Altogether, 1720 anther culture—derived green plantlets were produced from the four genotypes.
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Abbreviations
- DH:
-
Doubled haploid
- ELS:
-
Embryo-like structures
- IMC:
-
Isolated microspore culture
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Acknowledgements
The authors thank the support of scientific projects (Project Code: OTKA-K_16-K119835; name of project: Improvement of spelt wheat lines with low fermentable carbohydrate content (FODMAP) using modern and classical research methods). The experiments were interlocked with GINOP project (Project Number: GINOP-2.2.1-15-2016-00026) and supportment of Foundation of Szegedért. This project was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. The authors also thank the conscientious work of Csaba Ponta, Ferenc Markó and Sándor Vajasdi-Nagy. We would like to acknowledge valuable comments and grammatical corrections made by Allan Rattey (Canberra, Australia).
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JP designing experiment, LB serving plant material, CL and ÉN carrying out experiments and statistical analyses, CL FB and JP writing manuscript.
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Communicated by Sergio J. Ochatt.
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Lantos, C., Bóna, L., Nagy, É. et al. Induction of in vitro androgenesis in anther and isolated microspore culture of different spelt wheat (Triticum spelta L.) genotypes. Plant Cell Tiss Organ Cult 133, 385–393 (2018). https://doi.org/10.1007/s11240-018-1391-z
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DOI: https://doi.org/10.1007/s11240-018-1391-z