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Plant regeneration through culture of isolated microspores of Triticum aestivum L.

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Abstract

Wheat microspores mechanically isolated from the anthers before culture and isolated from the anthers during the hole culture period in a chemically defined medium resulted in proembryos, embryos and finally plants. Of the four genotypes included, all responded with proembryos, and the two spring wheats ‘Ciano’ and ‘Walter’ gave rise to macroscopic embryos and plants. The frequency of embryo regeneration and the frequency of albino plants in both ‘Ciano’ and ‘Walter’ was in accordance with previously obtained results with anther culture derived material.

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Abbreviations

2,4-d :

2,4-dichlorophenoxy acetic acid

NAA:

1-naphthaleneacetic acid

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Authors and Affiliations

  1. Nilsson-Ehle Laboratory, Svalöf AB, 268 81, Svalöv, Sweden

    Inger Kirstine Due Tuvesson & Rebecka Charlotte Viktoria Öhlund

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  1. Inger Kirstine Due Tuvesson
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  2. Rebecka Charlotte Viktoria Öhlund
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Due Tuvesson, I.K., Viktoria Öhlund, R.C. Plant regeneration through culture of isolated microspores of Triticum aestivum L.. Plant Cell Tiss Organ Cult 34, 163–167 (1993). https://doi.org/10.1007/BF00036097

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  • DOI: https://doi.org/10.1007/BF00036097

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