Summary
Wheat microspores were isolated, without prior anther culture, from a range of genotypes and cultured to regenerate self-fertile plants. Microspores were isolated using a microblender and competent microspores were enriched by gradient centrifugation. The use of maltose as the sole carbohydrate in the culture medium and co-culture of microspores with wheat or barley ovaries were critical for development of microspore-derived embryos. Results were also improved when spikes were pretreated by emersion of the basal ends of detached heads in water at 25°C for 2d. This procedure leads to highly reproducible production of plants.
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Communicated by J.J. Finer
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Mejza, S.J., Morgant, V., DiBona, D.E. et al. Plant regeneration from isolated microspores of Triticum aestivum . Plant Cell Reports 12, 149–153 (1993). https://doi.org/10.1007/BF00239096
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DOI: https://doi.org/10.1007/BF00239096