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Somatic embryogenesis of the heavy metal accumulator Prosopis laevigata

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Abstract

Somatic embryogenesis and whole plant regeneration was achieved in callus cultures derived from immature zygotic embryos of Prosopis laevigata (Humb. & Bonpl. ex Willd.) M.C. Johnst., recently identified as chromium (Cr), cadmium (Cd), lead (Pb) and nickel (Ni) accumulator. Embryogenic calli were induced on Murashige and Skoog (MS) medium added with a mixture of organic components plus N-6 benzyladenine (BA) (6.62 μM) and 2,4-dichlorophenoxyacetic acid (2,4-D) (2.26 μM) or thidiazuron (4.54–9.08 μM) and indole-3-acetic acid (1.42 μM). Embryogenic calli transferred onto half-strength MS medium without plant growth regulators developed globular embryos, of which 20% matured when treated with 3.75% (w/v) polyethylene glycol (PEG), and of these 50% fully differentiated into plantlet embryo. Regenerated plants were successfully acclimatized (90%), while in vitro seedlings transferred to MS medium containing 0.5 mM Cd, Cr, Ni or Pb, exhibited high heavy metals accumulation (627 mg Cr kg−1, 5,688 mg Cd kg−1, 1,148 mg Ni kg−1, and 3,037 mg Pb kg−1 dry weight) and efficient roots to shoots translocation (42–73%).

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Abbreviations

ABA:

Abscisic acid

AGP:

Arabinogalactan protein

Arg:

l-Arginine

Asn:

l-Asparagine monohydrate

BA:

N-6 Benzyladenine

BF:

Bioaccumulation factor

Cd:

Cadmium

CH:

Casein enzymatic hydrolysate

Cr:

Chromium

Cam:

Citric acid monohydrate

2,4-D:

2,4-Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

Gln:

Glutamine

HM:

Heavy metals

HDPE:

High density polyethylene

IAA:

Indole-3-acetic acid

Pb:

Lead

MS:

Murashige and Skoog (1962) medium

Ni:

Nickel

PEG:

Polyethylene glycol

PGR:

Plant growth regulator

PVP:

Polyvinylpyrrolidone

TBA:

Tertiary butyl alcohol

TDZ:

Thidiazuron

TF:

Translocation factor

Vc:

Vitamin C (L-ascorbic acid)

References

  • Alkhateeb A (2008) Comparison effects of sucrose and date palm syrup on somatic embryogenesis of date palm (Phoenix dactylifera L). Am J Biochem Biotechol 4:19–23

    Article  CAS  Google Scholar 

  • Baker CM, Burns JA, Wetzstein HY (1994) Influence of photoperiod and medium formulation of peanut somatic embryogenesis. Plant Cell Rep 13:159–163

    Article  CAS  Google Scholar 

  • Bellettrea A, Couillerota JP, Blervacqa AS, Aubertb S, Goutc E, Hilberta JL, Vasseura J (2001) Glycerol effects both carbohydrate metabolism and cytoskeletal rearrangements during the induction of somatic embryogenesis in chicory leaf tissues. Plant Physiol Biochem 39:503–511

    Article  Google Scholar 

  • Bonga JM, Klimaszewska KK, von Aderkas P (2010) Recalcitrance in clonal propagation, in particular of conifers. Plant Cell Tissue Organ Cult 100:241–254

    Article  Google Scholar 

  • Buendía-González L, Orozco-Villafuerte J, Cruz-Sosa F, Chávez-Ávila VM, Vernon-Carter EJ (2007) Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I. via cotyledonary nodes. In Vitro Cell Dev Biol Plant 43:260–266

    Article  Google Scholar 

  • Buendía-González L, Orozco-Villafuerte J, Cruz-Sosa F, Barrera-Díaz CE, Vernon-Carter EJ (2010a) Prosopis laevigata a potential chromium (VI) and cadmium (II) hyperaccumulator desert plant. Bioresour Technol 10:5862–5867

    Article  Google Scholar 

  • Buendía-González L, Orozco-Villafuerte J, Estrada-Zúñiga ME, Barrera-Díaz CE, Vernon-Carter EJ, Cruz-Sosa F (2010b) In vitro lead and nickel accumulation in mesquite (Prosopis laevigata) seedlings. Rev Mex Ing Quím 9:1–9

    Google Scholar 

  • Cabral GB, Carneiro VTC, Lacerda AL, do Valle CB, Martinelli AP, Dusi DMA (2011) Somatic embryogenesis and organogénesis in apomictic and sexual Brachiaria brizantha. Plant Cell Tiss Organ Cult. doi: 10.1007/s11240-011-9978-7

  • Capataz-Tafur J, Trejo-Tapia G, Rodríguez-Monroy M, Sepulveda-Jiménez G (2011) Arabinogalactan proteins are involved in cell aggregation of cell suspension cultures of Beta vulgaris L. Plant Cell Tiss Organ Cult 106:169–177

    Article  CAS  Google Scholar 

  • Clemens S, Palmgren MG, Kramer U (2002) A long way ahead: understanding and engineering plant metal accumulation. Trends Plant Sci 7:309–315

    Article  PubMed  CAS  Google Scholar 

  • Dewan A, Nanda K, Gupta SC (1992) In vitro micropropagation of Acacia nilotica subsp. Indica Brenan via cotyledonary nodes. Plant Cell Rep 12:18–21

    Article  Google Scholar 

  • Felker P (1981) Uses of tree legumes in semiarid regions. Econ Bot 35:174–186

    Article  Google Scholar 

  • Gairi A, Rashid A (2004) Direct differentiation of somatic embryos on different regions of intact seedlings of Azadirachta in response to thidiazuron. J Plant Physiol 161:1073–1077

    Article  PubMed  CAS  Google Scholar 

  • Iantcheva A, Vlahova M, Bakalova E, Kondorosi E, Elliott MC, Atanassov A (1999) Regeneration of diploid annual medics via direct somatic embryogenesis promoted by thidiazuron and benzylaminopurine. Plant Cell Rep 18:904–910

    Article  CAS  Google Scholar 

  • Jensen WA (1962) Botanical histochemistry. W. H. Freeman, San Francisco

    Google Scholar 

  • Kokubo A, Sakurai N, Kuraishi S, Takeda K (1991) Culm brittleness of barley (Hordeum vulgare L.) mutants is caused by smaller number of cellulose molecules in cell wall. Plant Physiol 97:509–514

    Article  PubMed  CAS  Google Scholar 

  • Konieczny R, Pilarska M, Tuleja M, Salaj T, Ilnicki T (2010) Somatic embryogenesis and plant regeneration in zygotic embryos of Trifolium nigrescens (Viv.). Plant Cell Tissue Organ Cult 100:123–130

    Article  Google Scholar 

  • Lakshmanan P, Taji A (2000) Somatic embryogenesis in leguminous plants. Plant Biol 2:136–148

    Article  CAS  Google Scholar 

  • Letarte J, Simion E, Miner M, Kasha KJ (2006) Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep 24:691–698

    Article  PubMed  CAS  Google Scholar 

  • Lu J, Vahala J, Pappinen A (2011) Involvement of ethylene in somatic embryogenesis in Scots pine (Pinus sylvestris L). Plant Cell Tiss Organ Cult. doi: 10.1007/s11240-011-9952-4

  • Ma G, Lü J, da Silva JAT, Zhang X, Zhao J (2011) Shoot organogénesis and somatic embryogenesis from leaf and shoot explats of Ochna integérrima (Lour). Plant Cell Tissue Organ Cult 104:157–162

    Article  CAS  Google Scholar 

  • Maldonado-Aguirre LJ, De la Garza FE (2000) El mezquite en México: Rasgos de importancia productiva y necesidades de desarrollo. In: Frías-Hernández JT, Olalde-Portugal V, Vernon-Carter EJ (eds) El mezquite árbol de usos múltiples. Estado actual del conocimiento en México. Universidad de Guanajuato, Guanajuato, pp 13–36

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murthy BNS, Murch SJ, Saxena PK (1995) Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. Physiol Plant 94:268–276

    Article  CAS  Google Scholar 

  • NCSS-number cruncher (2001) Statistical software. Kaysville, Utah

    Google Scholar 

  • Orozco-Villafuerte J, Cruz-Sosa F, Ponce-Alquicira E, Vernon-Carter EJ (2003) Mesquite gum: fractionation and characterization of the gum exuded from Prosopis laevigata obtained from plant tissue culture and from wild trees. Carbohydr Polym 54:327–333

    Article  CAS  Google Scholar 

  • Orozco-Villafuerte J, Buendía-González L, Cruz-Sosa F, Vernon-Carter EJ (2005) Increased mesquite gum formation in nodal explants cultures after treatment with a microbial biomass preparation. Plant Physiol Biochem 43:802–807

    Article  PubMed  CAS  Google Scholar 

  • Palmer CD, Keller WA (2011) Plant regeneration using immature zygotic embryos of Tribulus terrestris. Plant Cell Tissue Organ Cult 105:121–127

    Article  Google Scholar 

  • Pereira-Nieto AB, Pettolino F, Cruz-Silva CTA, Simas FF, Bacic A, AMdA Carneiro-Leão, Lacomini M, Maurer JBB (2007) Cashew-nut tree exudate gum: identification of an arabinogalactan-protein as a constituent of the gum and use on the stimulation of somatic embryogenesis. Plant Sci 173:468–477

    Article  Google Scholar 

  • Rai MK, Shekhawar NS, Harish, Gupta AK, Phulwaria M, Ram K, Jaiswal U (2011) The role of abscisic acid in plat tissue culture: a review of recent progress. Plant Cell Tissue Organ Cult. doi: 10.1007/s11240-011-9923-9

  • Salaj J, Petrovská B, Obert B, Pret’ová A (2005) Histological study of embryo-like structures initiated from hypocotyl segments of flax (Linum usitatissimum L.). Plant Cell Rep 24:590–595

    Article  PubMed  CAS  Google Scholar 

  • Shoji M, Sato H, Nakagawa R, Funada R, Kubo T, Ogita S (2006) Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora. J For Res 11:449–453

    Article  Google Scholar 

  • Showalter AM (2001) Arabinogalactan-proteins: structure, expression and function. Cell Mol Life Sci 58:1399–1417

    Article  PubMed  CAS  Google Scholar 

  • Stanton J, Olson DK, Brock JH, Gordon RS (2001) The environmental and economic feasibility of alternative crops in arid areas: considering mesquite in Baja California, Mexico. J Arid Environ 48:9–22

    Article  Google Scholar 

  • Trejo-Espino JL, Rodríguez-Monroy M, Vernon-Carter EJ, Cruz-Sosa F (2010) Emulsifying properties of the gum produced by Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. (Mesquite) cells suspension culture in bioreactor. Rev Mex Ing Quim 9:251–260

    CAS  Google Scholar 

  • Xie DY, Hong Y (2001) Regeneration of Acacia mangium through somatic embryogenesis. Plant Cell Rep 20:34–40

    Article  CAS  Google Scholar 

  • Yang JL, Niu YD, Yang CP, Liu GF, Li CH (2011) Induction of somatic embryogenesis from female flower buds of elite Schisandra chinensis. Plant Cell Tissue Organ Cult. doi: 10.1007/s11240-011-9935-5

  • Yantcheva A, Vlahova M, Antanassov A (1998) Direct somatic embryogenesis and plant regeneration of carnation (Dianthus caryophyllus L.). Plant Cell Rep 18:148–153

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Facultad de Ciencias, Universidad Autónoma del Estado de México, Campus El Cerrillo, Piedras Blancas, Carretera Toluca-Ixtlahuaca km. 15.5, CP 50200, Toluca, Estado de México, Mexico

    L. Buendía-González

  2. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, Iztapalapa, 09340, Mexico, DF, Mexico

    M. E. Estrada-Zúñiga & F. Cruz-Sosa

  3. Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón esq. Paseo Tollocan s/n, Col. Residencial Colón, 50120, Toluca, Estado de México, Mexico

    M. E. Estrada-Zúñiga & J. Orozco-Villafuerte

  4. Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, 09340, Mexico, DF, Mexico

    E. J. Vernon-Carter

Authors
  1. L. Buendía-González
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  2. M. E. Estrada-Zúñiga
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  3. J. Orozco-Villafuerte
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  4. F. Cruz-Sosa
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  5. E. J. Vernon-Carter
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Correspondence to J. Orozco-Villafuerte.

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Buendía-González, L., Estrada-Zúñiga, M.E., Orozco-Villafuerte, J. et al. Somatic embryogenesis of the heavy metal accumulator Prosopis laevigata . Plant Cell Tiss Organ Cult 108, 287–296 (2012). https://doi.org/10.1007/s11240-011-0042-4

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  • DOI: https://doi.org/10.1007/s11240-011-0042-4

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