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Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

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Abstract

Levels of ethylene and polyamines (PAs) were measured during organogenesis of hypocotyl explants of two species of passion fruit (Passiflora cincinnata Masters and Passiflora edulis Sims f. flavicarpa Degener ‘FB-100’) to better understand the relationships of these regulators and their influence on cell differentiation and morphogenesis. Moreover, histological investigation of shoot ontogenesis was conducted to characterize the different events involved in cell redifferentiation and regulation of PA and ethylene levels. A delay was observed in morphogenic responses of P. edulis f. flavicarpa as compared to P. cincinnata, and these changes coincided with production of elevated levels of polyamine and ethylene levels. During differentiation, cells showed high rates of expansion and elongation, and high ethylene levels were associated with high PA levels, suggesting that the two biosynthesis pathways were highly regulated. Moreover, their interaction might be an important factor for determining cell differentiation. The addition of PAs to the culture medium did not promote organogenesis; however, the incorporation of the PA inhibitor methylglyoxal bisguanylhydrazone in the culture medium reduced shoot bud differentiation, suggesting the need to maintaining a minimum level of PAs for morphogenic events to take place.

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Acknowledgments

The authors thank Viveiros Flora Brasil Ltda (Araguari, MG, Brazil) for generously providing ‘FB-100’ Maguary seed population, FAPEMIG (Belo Horizonte, MG, Brazil), CNPq (Brasília, DF, Brazil), and CAPES (Brasília, DF, Brazil), for financial support. The authors are also grateful to the anonymous reviewers and to Dr. Schuyler S. Korban (Editor-in-Chief) for the criticisms and valuable suggestions for the manuscript.

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  1. Plant Cell Biology Laboratory (BIOCEL), Institute of Biological Sciences, Botany Department, University of São Paulo (USP), São Paulo, SP, 11461, Brazil

    Leonardo L. C. Dias & Eny I. S. Floh

  2. Cell Biology and Tissue Culture Laboratory, Biosciences and Biotechnology Center, North Fluminense Darcy Ribeiro State University (UENF), Campos dos Goytacazes, RJ, 28013-602, Brazil

    Claudete Santa-Catarina

  3. Plant Growth and Development Laboratory, Plant Biology Department, Federal University of Viçosa (UFV), Viçosa, MG, 36570-000, Brazil

    Dimas M. Ribeiro & Raimundo S. Barros

  4. Plant Tissue Culture Laboratory, Plant Biology Department/BIOAGRO, Federal University of Viçosa, Av. Peter Henry Rofs s/n, University Campus, Viçosa, MG, 36570-000, Brazil

    Wagner C. Otoni

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  1. Leonardo L. C. Dias
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Correspondence to Wagner C. Otoni.

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Dias, L.L.C., Santa-Catarina, C., Ribeiro, D.M. et al. Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor. Plant Cell Tiss Organ Cult 99, 199–208 (2009). https://doi.org/10.1007/s11240-009-9594-y

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