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Role of polyamines in adventitious shoot morphogenesis from cotyledons of cucumber in vitro

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Abstract

The relationship between polyamines (PAs) metabolism and adventitious shoot morphogenesis from cotyledons of cucumber was investigated in vitro. The endogenous levels of free putrescine (Put) and spermidine (Spd) in the explants decreased sharply, whereas endogenous spermine (Spm) increased during adventitious shoot morphogenesis. The presence of 1–15 mM Put, 1–2 mM Spd, 0.05–1 mM Spm, 5–10 μM aminoethoxyvinylglycine (AVG) or 5 μM AVG together with 50 μM 1-aminocyclopropane-1-carboxylic acid (ACC) in the regeneration medium could promote adventitious shoot formation. Conversely, 1–5 mM D-arginine (D-Arg) or 0.01–0.1 mM methylglyoxal bis-guganylhydrazone (MGBG) inhibited regeneration; and 0.005–0.05 mM ACC displayed little or no evident effects. The explants growing on medium containing 5 μM AVG produced higher levels of free Put and Spm, and on medium containing 5 mM Put the explants responded similarly to the AVG-treated explants. However, the exogenous use of 1 mM D-Arg reduced the levels of Put, Spd and Spm, and 0.1 mM MGBG reduced the levels of free Spd and Spm. Moreover, although the explants cultured on medium containing Put and MGBG enhanced ethylene production, AVG and D-Arg inhibited ethylene biosynthesis. This study shows the PAs requirement for the formation of adventitious shoot from cotyledons of cucumber in vitro and the enhanced adventitious shoot morphogenesis may be associated with the elevated level of endogenous free Spm, albeit the promotive effect of PAs on adventitious shoot morphogenesis may not be related to ethylene metabolism.

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Abbreviations

AVG:

aminoethoxyvinylglycine

D-Arg:

D-arginine

KT:

kinetin

NAA:

α-naphthaleneacetic acid

PAs:

polyamines

Put:

putrescine

SAM:

S-adenosylmethionine

Spd:

spermidine

Spm:

spermine

MGBG:

methylglyoxal-bis-guganylhydrazone

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  1. College of Life Sicences, Zhejiang University, Hangzhou, Zhejiang Province, 310029, China

    Cheng Zhu & Zhen Chen

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  1. Cheng Zhu
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  2. Zhen Chen
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Correspondence to Cheng Zhu.

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Zhu, C., Chen, Z. Role of polyamines in adventitious shoot morphogenesis from cotyledons of cucumber in vitro. Plant Cell Tiss Organ Cult 81, 45–53 (2005). https://doi.org/10.1007/s11240-004-2773-y

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