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Assessment of the polyamines modulation on cytokinins and ethylene and its effect in lemon (Citrus limon) de novo regeneration

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Abstract

A highly productive and robust adventitious organogenesis protocol is key as an initial step when lemon in vitro mutagenesis or genetic transformation are addressed. Many molecules play a major role on plant regeneration, and in this study, the addition of the main naturally-occurring polyamines (spermine, spermidine, and putrescine) and their effect on endogenous cytokinins and on ethylene production have been analyzed in explants of the lemon (Citrus limon) cultivar ‘Verna 51’.The three polyamines were added to the organogenesis media, and the regeneration rate and percentage were recorded. Changes produced in the endogenous cytokinins along with ethylene production were studied. Polyamines were shown to play an important role in the adventitious shoot regeneration of ‘Verna 51’. However, this effect would not be related to ethylene production since changes were not detected. Comparing the three studied polyamines, it is possible to conclude that, within the studied doses, spermine (0.2 mM) and spermidine (0.3 and 0.6 mM) are more useful to induce organogenesis than putrescine in ‘Verna 51’. In the most regenerative treatments, lower concentrations of cytokinins were found, which could indicate a correlation between the addition of polyamines to the culture media, the adventitious regeneration, and the metabolism of cytokinins.

Key message

Polyamines are able to modulate de novo plant regeneration through changes produced in the endogenous cytokinins while do not appear to interfere directly in the ethylene production in lemon tissues.

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Data Availability

The data generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors want to thank Fernando Córdoba for his technical assistance in the laboratory.

Funding

This work was supported by the European Regional Development Fund.

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Authors and Affiliations

  1. Equipo de Mejora Genética de Cítricos, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150, Murcia, Spain

    Margarita Pérez-Jiménez, Virginia Celdrán-Sánchez & Olaya Pérez-Tornero

  2. Departamento de Tecnología Alimentaria, Universidad Miguel Hernández, Ctra. de Beniel km. 3.2, 03312, Alicante, Orihuela, Spain

    Domingo Martínez-Romero

Authors
  1. Margarita Pérez-Jiménez
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  2. Virginia Celdrán-Sánchez
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  3. Domingo Martínez-Romero
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  4. Olaya Pérez-Tornero
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Contributions

OPT and MPJ conceived the study. VCS conducted the experiments. MPJ conducted the endogenous cytokinins analysis. DMR conducted the ethylene analysis. OPT and MPJ analyzed the experimental data, interpreted the results and prepared the original draft. OPT was responsible for funding acquisition and project administration. All authors read and approved the manuscript.

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Correspondence to Margarita Pérez-Jiménez.

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The authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest.

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Communicated by Klaus Eimert.

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Pérez-Jiménez, M., Celdrán-Sánchez, V., Martínez-Romero, D. et al. Assessment of the polyamines modulation on cytokinins and ethylene and its effect in lemon (Citrus limon) de novo regeneration. Plant Cell Tiss Organ Cult 150, 165–175 (2022). https://doi.org/10.1007/s11240-022-02257-w

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  • DOI: https://doi.org/10.1007/s11240-022-02257-w

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