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The influence of ethylene in plant tissue culture

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Abstract

Ethylene produced by plant tissues grown in vitro may accumulate in large quantities in the culture vessels, particularly from rapidly growing non-differentiated callus or suspension cultures, and hence is likely to influence growth and development in such systems. Research into this aspect of tissue culture has been sparse, although it has grown recently with the increasing importance of in vitro regeneration. This review deals with the measurement and relevance of the accumulated ethylene, and the influence of both exogenous and endogenous ethylene in the different types of tissue culture systems. The relationships between ethylene and other growth regulators in tissue culture growth and development are also discussed. Although in some cases its influence seems negligible, in many types of tissue culture ethylene may act either as a promoter or inhibitor depending on the species used. Thus ethylene has an important influence on many aspects of in vitro regeneration, but it is also clear that we cannot at present describe a specific role or roles for ethylene in tissue culture which can be applied at a general, species-wide level. If its effects are to be enhanced or diminished in order to improve the efficiency and range of plant tissue culture, then more research is needed to clarify what its fundamental role might be in in vitro growth and development.

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Abbreviations

ABA:

abscisic acid

ACC:

1-aminocyclopropane-1-carboxylic acid

AOA:

aminooxyacetic acid

ASA:

acetylsalicyclic acid

AVG:

aminoethoxyvinylglycine

BA:

N6 benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid

DNP:

2,4-dinitrophenol

GA:

gibberellin

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

NAA:

naphthaleneacetic acid

SAM:

S-adenosylmethionine

STS:

silver thiosulphate

TIBA:

2,3,5-triidobenzoic acid

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Biddington, N.L. The influence of ethylene in plant tissue culture. Plant Growth Regul 11, 173–187 (1992). https://doi.org/10.1007/BF00024072

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