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Efficient plant regeneration from long-term callus cultures of rice by spermidine

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Abstract

A significant reduction in regeneration potential with increasing age (upto 12months) in rice (Oryza sativa L. cv.TN-1) embryogenic callus cultures was observed. Spermidine, while having an inhibitory effect on plant regeneration in fresh callus cultures, promoted morphogenesis in long-term callus cultures. A massive accumulation of polyamines, particularly putrescine (5-fold) was observed in 12 month old cultures resulting in a change of putrescine /spermidine ratio, which seems to be important for maintaining the morphogenetic response. Application of exogenous spermidine to 12 month old cultures showed increased levels of polyamines and restored the putrescine/spermidine ratio comparable to that found in freshly induced cultures, concomitantly, promoting the plant regeneration via somatic embryogenesis in long-term rice callus cultures.

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Abbreviations

PA:

Polyamines

PCA:

Perchloric acid

PUT:

Putrescine

SPD:

Spermidine

SPM:

Spermine

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Authors and Affiliations

  1. Department of Genetics, University of Delhi, South Campus, Benito Juarez Road, -110021, New Delhi, India

    S. Bajaj & M. V. Rajam

Authors
  1. S. Bajaj
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  2. M. V. Rajam
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Communicated by A. Komamine

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Bajaj, S., Rajam, M.V. Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell Reports 14, 717–720 (1995). https://doi.org/10.1007/BF00232654

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  • DOI: https://doi.org/10.1007/BF00232654

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