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Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

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Abstract

Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks.

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  1. Facultad de Ciencias Químicas, Universidad Veracruzana, Prolongación Oriente. 6, No. 1009, Orizaba, Veracruz, CP 94340, Mexico

    Maria Teresa Gonzalez-Arnao

  2. Centro Internacional de la Papa (CIP), Apartado 1558, Lima, 12, Peru

    Ana Panta & William M. Roca

  3. Centro Internacional de Agricultura Tropical (CIAT), Apartado Aereo 6713, Cali, Colombia

    Roosevelt H. Escobar

  4. Institut de recherche pour le développement (IRD), UMR DIA-PC, 911 avenue Agropolis, BP 64501, Montpellier Cedex 5, 34394, France

    Florent Engelmann

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  1. Maria Teresa Gonzalez-Arnao
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  2. Ana Panta
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Correspondence to Maria Teresa Gonzalez-Arnao.

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Gonzalez-Arnao, M.T., Panta, A., Roca, W.M. et al. Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops. Plant Cell Tiss Organ Cult 92, 1–13 (2008). https://doi.org/10.1007/s11240-007-9303-7

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