Abstract
Shoot tips isolated from a mint clone, Mentha aquatica x M. spicata, were gradually exposed to a mixture containing 35% ethylene glycol, 1 M dimethylsulfoxide and 10% polyethylene glycol-8000 and then immersed into liquid nitrogen. Cooling and warming rates were approximately 4800°C/min and 9000°C/min respectively. Survival after liquid nitrogen treatment ranged from 31% to 75% among experiments. There was no obvious reason for this variation. In many cases the treated shoot tip directly developed into a shoot without any or with only slight callus formation.
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Abbreviations
- DSC:
-
differential scanning calorimetry
- DMSO:
-
dimethylsulfoxide
- EG:
-
ethylene glycol
- PEG-8000:
-
polyethylene glycol
- MW:
-
avg. 8000
- LN:
-
liquid nitrogen
- IBA:
-
indolebutyric acid
- BA:
-
benzyladenine
References
Fahy GM, Levy DI and Ali SE (1987) Cryobiology 24:196–213
Fahy GM (1988) In: McGrath JJ and Diller KR (eds) Low temperature biotechnology, American Society of Mechanical Engineers, New York, New York, pp 113–146
Grout BWW, Henshaw GG (1978) Ann. Bot. 1227–1229
Langis R, Schnabel B, Earle ED and Steponkus PL (1989) Cryo-Letters 10:421–428
Langis R, and Steponkus PL (1990) Plant Physiol 92:666–671
Murashige, T and Skoog F (1962) Physiol Plant 15:473–497
Seibert M, Wetherbee PJ (1977) Plant Physiol 59:1043–1046
Stanwood PC (1985) In: Kartha KK (ed) Cryopreservation of plant cells and organs, CRC Press, Inc. Boca Raton, Florida, pp 199–226
Towill LE (1985) In: Kartha KK (ed) Cryopreservation of plant cells and organs, CRC Press, Inc. Boca Raton, Florida, pp 171–198
Towill LE (1988) HortScience 23:839–841
Towill LE, Roos EE (1989) In: Knutson L and Stoner AK (eds) Biotic diversity and germplasm preservation, global imperatives, Kluwer Academic Publishers, Dordrecht, pp 379–403
Towill LE (1990) In: Dodds JH (ed) Tissue culture methods for conservation of plant genetic resources, Van Nostrand Reinhold, in press
Uragami A, Sakai A, Nagai M and Takahashi T (1989) Plant Cell Reports 8:418–421
Withers LA (1987) In: Grout BWW and Morris GJ (eds) The effects of low temperatures on biological systems, Arnold Publishers, London, pp 389–409
Withers LA, Benson EE and Martin M (1988) Cryo-Letters 9:114–119
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Communicated by G. C. Phillips
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Towill, L.E. Cryopreservation of isolated mint shoot tips by vitrification. Plant Cell Reports 9, 178–180 (1990). https://doi.org/10.1007/BF00232174
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DOI: https://doi.org/10.1007/BF00232174