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Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification

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Summary

Alginate coated meristems from in vitro-grown axillary buds of potato (Solanum tuberosum L.) were successfully cryopreserved by vitrification. Excised meristems were precultured on sucrose-enriched MS medium and then encapsulated. To induce dehydration tolerance (osmotolerance), encapsulated meristems were treated with a mixture of 2 M glycerol plus 0.6 M sucrose for 90 min. These encapsulated meristems were dehydrated with a highly concentrated vitrification solution (PVS2 solution) for 3 hr at 0°C prior to a plunge into liquid nitrogen. Successfully vitrified meristems developed shoots within 3 weeks after plating without intermediary callus formation. The average rate of shoot formation amounted to nearly 70%. No difference was observed in RAPD analysis using 17 primers between cryopreserved and non-treated plantlets. The cryogenic protocol was successfully applied to 14 cultivars. It was also confirmed that the encapsulated vitrified meristems produced much greater shoot formation than the encapsulated dried meristems. Thus, the encapsulation vitrification protocol appears promising for cryopreservation of potato germplasm.

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Authors and Affiliations

  1. Hokkaido Prefectural Plant Genetic Resources Center, 363-2, Minami-takinokawa, 073-0013, Takikawa, Hokkaido, Japan

    Dai Hirai

  2. 1-5-23, Asabu-cho, Kita-ku, 001-0045, Sapporo, Hokkaido, Japan

    Akira Sakai

Authors
  1. Dai Hirai
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  2. Akira Sakai
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Hirai, D., Sakai, A. Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification. Potato Res 42, 153–160 (1999). https://doi.org/10.1007/BF02358405

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