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Survival of somatic embryos and recovery of plants of sweet orange (Citrus sinensis (L.) Osb.) after immersion in liquid nitrogen

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Abstract

Somatic embryos of ‘Washington Navel’ sweet orange (Citrus sinensis (L.) Osb.) derived from in vitro cultured ovules excised from immature fruits, were frozen to the temperature of liquid nitrogen. A method of slow cooling at a rate of 0.5°C min-1 down to −42°C followed by storage in liquid nitrogen was used. Thawing was achieved by keeping the specimens at room temperature for 15 min. A small number of frozen embryos survived and developed into proliferating cultures that produced whole plants. The plants obtained from frozen cultures were transferred to soil and are growing successfully.

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References

  1. Bajaj YPS (1979) Establishment of germplasm banks through freeze-storage of plant tissue culture and their implications in agriculture. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (Eds) Plant Cell and Tisue Culture—Principles and Applications, pp 745–774. Ohio State University Press, Columbus, Ohio

    Google Scholar 

  2. Bajaj YPS (1984) Induction of growth in frozen embryos of coconut and ovules of Citrus. Curr Sci 53 (22): 1215–1216

    Google Scholar 

  3. Bajaj YPS (1985) Cryopreservation of embryos. In: Kartha KK (Ed) Cryopreservation of Plants, Cells and Organs, pp 227–242. CRC Press, Boca Raton, Florida

    Google Scholar 

  4. Bitters WP, Murashige T, Rangan TS, Nauer E (1972) Investigations on established virus-free citrus plants through tissue culture. In: Price WC (Ed) Proc 5th Conf Inter Organ Citrus Virol, pp 267–271. University of Florida Press, Gainesville, Florida

    Google Scholar 

  5. Button J, Bornman CM (1971) Development of nucellar plants from unpollinated and unfertilized ovules of the Washington Navel orange in vitro. J S Afr Bot 37 (2): 127–134

    Google Scholar 

  6. Kartha KK (1985) Cryopreservation of plant cells and organs. IAPTC Newsletter 45: 2–15

    Google Scholar 

  7. Kartha KK (1985) Meristem culture and germplasm preservation. In: Kartha K.K. (Ed) Cryopreservation of Plant Cells and Organs, pp 115–134. CRC Press, Boca Raton, Florida

    Google Scholar 

  8. Katano M, Ishihara A, Sakai A (1983). Survival of dormant apple shoot tips after immersion in liquid nitrogen. HortSci 18 (5): 707–708

    Google Scholar 

  9. King MW, Soetisna V, Roberts EH (1981) The dry storage of Citrus seeds. Ann Bot 48: 865–872

    Google Scholar 

  10. Mumford PM, Panggabean G (1982) A comparison of the effect of dry storage on seeds of Citrus species. Seed Sci Technol 10: 257–266

    Google Scholar 

  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  12. Navarro L, Juarez J (1977) Tissue culture techniques used in Spain to recover virus-free citrus plants. Acta Hortic 78: 425–435

    Google Scholar 

  13. Navarro L, Juarez J, Ballester JF, Pina JA Ortega C (1979) Obtencion de plantas nucelares libres de virus de diversas variedades de agrios del grupo Navel (C. sinensis (L.) Osbeck) por cultivo de ovulos in vitro. Anales de INIA, Serie Proteccion Vegetal, No 12, pp 19

  14. Sabharwal PS (1963) In vitro culture of ovules, nucelli and embryos of Citrus reticulata Blanco var Nagpuri. In: Maheshawari P, Rangaswamy NS (Eds) Plant Tissue Organ Culture, pp 265–273. Symposium, University of Delhi, India

    Google Scholar 

  15. Sakai A, Sugawara Y (1973) Survival of poplar callus at super-low temperatures after cold acclimatation. Plant Cell Physiol 14: 1201–1204

    Google Scholar 

  16. Sakai A (1986) Cryoconservation of germplasm of woody plants. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry. Trees I, pp 113–129. Springer-Verlag, Berlin/ Heidelberg/New York/Tokyo

    Google Scholar 

  17. Towil LM, Mazur P (1976) Osmotic shrinkage as a factor in freezing injury in plant tissue cultures. Plant Physiol 57: 290–296

    Google Scholar 

  18. Withers LA (1979) Freeze-preservation of somatic embryos and clonal plantlets of carrot (Daucus carota L.) Plant Physiol 63: 460–467

    Google Scholar 

  19. Withers LA (1980) Storage of plant tissue cultures. In: Withers LA, Williams JT (Eds) Crop Genetic Resources the Conservation of Difficult Material. Proceedings of an International Workshop, University of Reading, UK. IUBS Serie B42 (IBPGR), Paris, pp 49–82

    Google Scholar 

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Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial, 46113, Moncada, (Valencia), Spain

    M. L. Marin & N. Duran-Vila

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  1. M. L. Marin
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  2. N. Duran-Vila
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Marin, M.L., Duran-Vila, N. Survival of somatic embryos and recovery of plants of sweet orange (Citrus sinensis (L.) Osb.) after immersion in liquid nitrogen. Plant Cell Tiss Organ Cult 14, 51–57 (1988). https://doi.org/10.1007/BF00029575

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  • DOI: https://doi.org/10.1007/BF00029575

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