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Cryopreservation of oil palm (Elaeis guineensis Jacq.) somatic embryos involving a desiccation step

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Summary

The standard cryopreservation process previously developed for oil palm clones using shiny white, finger-like somatic embryos could be applied in some cases to standard cultures. Its efficiency was markedly improved by completing the 7-day pregrowth period on 0.75 M sucrose by an additional dehydration period carried out either by placing the embryos in the air current of the laminar flow cabinet or in an air tight box containing silica gel. This improved process was successfully applied to 7 different clones. It will facilitate the routine uof cryopreservation for oil palm cultures.

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Authors and Affiliations

  1. Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, ORSTOM, 911, av. Agropolis, B.P. 5045, 34032, 01, Montpellier Cedex, France

    Dominique Dumet, Florent Engelmann, Nathalie Chabrillange & Yves Duval

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  1. Dominique Dumet
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  2. Florent Engelmann
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  3. Nathalie Chabrillange
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  4. Yves Duval
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Communicated by A. M. Boudet

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Dumet, D., Engelmann, F., Chabrillange, N. et al. Cryopreservation of oil palm (Elaeis guineensis Jacq.) somatic embryos involving a desiccation step. Plant Cell Reports 12, 352–355 (1993). https://doi.org/10.1007/BF00237434

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  • DOI: https://doi.org/10.1007/BF00237434

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