Skip to main content
SpringerLink
Log in

Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The effects of jasmonic acid (JA) and methyl jasmonate (MeJA) on potato (Solanum tuberosum L.) tuberization were studied in relation to cytokinins using single-node cuttings (SNCs) in vitro. In three potato cultivars differing in maturity levels, JA or MeJA (0.0, 2.5, 5.0, 7.5 and 10.0 μM) action was examined with N6-benzyladenine (BA: 0.0, 22.0 and 44.0 μM) under optimum tuber-inducing treatment with 80 g L−1 sucrose. Although jasmonates had a stimulatory role in root growth from SNCs, BA inhibited the root growth and antagonized the jasmonates action on root growth promotion. There was a strong inhibitory effect of BA on stoloniferous shoot growth, and in combination with jasmonates it could exert a much pronounced inhibitory effect. Jasmonates did not have any role in tuber induction in terms of tuber number and source-sink coordination (harvest index), rather their effects were counteracted in the presence of 22.0 μM BA in early cultivar. However, they had a promoting effect on tuber growth after induction in early cultivar, possibly due to its lower levels of endogenous gibberellins. Cytokinins detrimentally affected the tuber growth, and antagonized the jasmonates action irrespective of the maturity levels of the cultivars. It could, however, increase the tuber dry matter concomitant with a higher accumulation of starch. The promoting effect of jasmonates on tuber dry matter and starch accumulation was apparent only in early cultivar. There were significant interactions between cytokinin and jasmonates for accumulation of reducing and total sugars in tubers. The results show that exogenous cytokinins antagonize the jasmonates action, and the correlated effects of these two growth hormones interact with the maturing time of the cultivar during potato tuber formation in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

BA:

N6-benzyladenine

JA:

Jasmonic acid

KA:

Kufri Ashoka

KBD:

Kufri Badshah

KS:

Kufri Sindhuri

MeJA:

Methyl jasmonate

MS:

Murashige and Skoog’s medium (1962)

SNC:

Single-node cutting

References

  • Abdala G, Castro G, Guiňazŭ R, Tizio R, Miersch O (1996) Occurrence of jasmonic acid in organs of Solanum tuberosum L. and its effect on tuberization. Plant Growth Regul 19:139–143

    Article  CAS  Google Scholar 

  • Castro G, Abdala G, Agüero C, Tizio R (2000) Interaction between jasmonic and gibberellic acids on in vitro tuberization of potato plantlets. Potato Res 43:83–88

    Article  CAS  Google Scholar 

  • Dermastia M, Ravnikar M, Vilhar B, Kovac M (1994) Increased level of cytokinin ribosides in jasmonic acid-treated potato (Solanum tuberosum) stem node cultures. Physiol Plant 92:241–246

    Article  CAS  Google Scholar 

  • Dermastia M, Ravnikar M, Kovac M (1996) Morphology of potato (Solanum tuberosum L. cv. Sante) stem node cultures in relation to the level of endogenous cytokinins. J Plant Growth Regul 15:105–108

    Article  CAS  Google Scholar 

  • Donnelly DJ, Coleman WK, Coleman SE (2003) Potato microtuber production and performance: a review. Am J Potato Res 80:103–115

    Google Scholar 

  • Estrada R, Tovar P, Dodds JH (1986) Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell Tiss Org Cult 7:3–10

    Article  Google Scholar 

  • Helder H, Miersch O, Vreugdenhil D, Sembdner G (1993) Occurrence of hydroxylated jasmonic acids in leaflets of Solanum demissum plants grown under long- and short-day conditions. Physiol Plant 88:647–653

    Article  CAS  Google Scholar 

  • Hussey G, Stacey NJ (1984) Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Ann Bot 53:565–578

    CAS  Google Scholar 

  • Jackson SD (1999) Multiple signalling pathways control tuber induction in potato. Plant Physiol 119:1–8

    Article  PubMed  CAS  Google Scholar 

  • Jackson SD, Willmitzer L (1994) Jasmonic acid spraying does not induce tuberization in short-day requiring potato species kept in non-inducing conditions. Planta 194:155–159

    Article  CAS  Google Scholar 

  • Jásik J, de Klerk G-J (2006) Effect of methyl jasmonate on morphology and dormancy development in lily bulbets regenerated in vitro. J Plant Growth Regul 25:45–51

    Article  CAS  Google Scholar 

  • Koda Y (1997) Possible involvement of jasmonates in various morphogenic events. Physiol Plant 100:639–646

    Article  CAS  Google Scholar 

  • Koda Y, Kikuta Y (2001) Effects of jasmonates on in vitro tuberization in several potato cultivars that differ greatly in maturity. Plant Prod Sci 4:66–70

    Article  CAS  Google Scholar 

  • Koda Y, Okazawa Y (1988) Detection of potato tuber-inducing activity in potato leaves and old tubers. Plant Cell Physiol 29:1047–1051

    CAS  Google Scholar 

  • Koda Y, Kikuta Y, Takazi H, Tsujino Y, Sakamura S, Yoshihara T (1991) Potato tuber-inducing activities of jasmonic acid and related compounds. Phytochemistry 30:1435–1438

    Article  CAS  Google Scholar 

  • Mithöfer A, Maitrejean M, Boland W (2005) Structural and biological diversity of cyclic octadecanoids, jasmonates and mimetics. J Plant Growth Regul 23:170–178

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Naik PS, Sarkar D, Gaur PC (1998) Yield components of potato microtubers: in vitro production and field performance. Ann Appl Biol 133:91–99

    Google Scholar 

  • Palmer CE, Smith OE (1969) Cytokinins and tuber initiation in the potato Solanum tuberosum L. Nature 221:279–280

    Article  CAS  Google Scholar 

  • Pelacho AM, Mingo-Castel AM (1991) Jasmonic acid induces tuberization of potato stolons cultured in vitro. Plant Physiol 97:1253–1255

    Article  PubMed  CAS  Google Scholar 

  • Pruski K, Duplessis P, Lewis T, Astatkie T, Nowak J, Struik PC (2001) Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions. Potato Res 44:315–325

    Article  CAS  Google Scholar 

  • Pruski K, Astatkie T, Nowak J (2002) Jasmonate effects on in vitro tuberization and tuber bulking in two potato cultivars (Solanum tuberosum L.) under different media and photoperiod conditions. In Vitro Cell Develop Biol Plant 38:203–209

    Article  CAS  Google Scholar 

  • Ravnikar M, Vilhar B, Gogala N (1992) Stimulatory effects of jasmonic acid on potato node and protoplast culture. J Plant Growth Regul 11:29–33

    Article  CAS  Google Scholar 

  • Sarkar D, Naik PS (1998) Effect of inorganic nitrogen nutrition on cytokinin-induced potato microtuber production in vitro. Potato Res 41:211–217

    Article  CAS  Google Scholar 

  • Sarkar D, Chandra R, Naik PS (1997) Effect of inoculation density on potato micropropagation. Plant Cell Tiss Org Cult 48:63–66

    Article  CAS  Google Scholar 

  • Sarkar D, Pandey SK, Sud KC, Chanemougasoundharam A (2004) In vitro characterization of manganese toxicity in relation to phosphorous nutrition in potato (Solanum tuberosum L.). Plant Sci 167:977–986

    Article  CAS  Google Scholar 

  • Seabrook JEA, Douglass LK, Arnold DA (2004) Effect of leaves on microtubers produced from potato single-node cuttings in vitro. Am J Potato Res 81:1–5

    Article  Google Scholar 

  • Sharma S, Chanemougasoundharam A, Sarkar D, Pandey SK (2004) Carboxylic acids affect induction, development and quality of potato (Solanum tuberosum L.) microtubers grown in vitro from single-node explants. Plant Growth Regul 44:219–229

    Article  CAS  Google Scholar 

  • Slimmon T, Souza Machado V, Coffin R (1989) The effect of light on in vitro microtuberization of potato cultivars. Am Potato J 66:843–848

    Google Scholar 

  • van den Berg JH, Ewing EE (1991) Jasmonates and their role in plant growth and development, with special reference to the control of potato tuberization: a review. Am Potato J 68:781–794

    Google Scholar 

  • Veramendi J, Willmitzer L, Trethewey RN (1999) In vitro grown potato microtubers are a suitable system for the study of primary carbohydrate metabolism. Plant Physiol Biochem 37:693–697

    Article  CAS  Google Scholar 

  • Vreugdenhil D (2004) Comparing potato tuberization and sprouting: opposite phenomena. Am J Potato Res 81:275–280

    CAS  Google Scholar 

  • Vreugdenhil D, Struik PC (1989) An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum). Physiol Plant 75:525–531

    Article  CAS  Google Scholar 

  • Wang PJ, Hu CY (1982) In vitro mass tuberization and virus-free seed potato production in Taiwan. Am Potato J 59:33–39

    Google Scholar 

  • Wasternack C, Hause B (2002) Jasmonates and octadecanoids. Progr Nucleic Acid Res 72:165–221

    Article  CAS  Google Scholar 

  • Wattimena GA (1983) Micropropagation as an alternative technology for potato production in Indonesia. PhD dissertation, University of Wisconsin, Madison

  • Xiong X, Tai GCC, Seabrook JEA (2002) Effectiveness of selection for quality traits during the early stage in potato breeding population. Plant Breed 121:441–444

    Article  Google Scholar 

  • Xu X, van Lammeren AAM, Vermeer E, Vreugdenhil D (1998) The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol 117:575–584

    Article  PubMed  CAS  Google Scholar 

  • Yoshihara T, Omer E-LA, Koshino H, Sakamura S, Kikuta Y, Koda Y (1989) Structure of a tuber-inducing stimulus from potato leaves (Solanum tuberosum L.). Agr Biol Chem 53:2835–2837

    CAS  Google Scholar 

Download references

Acknowledgements

Comments and suggestions on the manuscript from the Editor and two anonymous reviewers are gratefully acknowledged.

Author information

Authors and Affiliations

  1. Division of Crop Improvement, Central Potato Research Institute, Shimla, 171 001, Himachal Pradesh, India

    Debabrata Sarkar, Suman Kumar Pandey & Sushruti Sharma

Authors
  1. Debabrata Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Suman Kumar Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sushruti Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Debabrata Sarkar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sarkar, D., Pandey, S.K. & Sharma, S. Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell Tiss Organ Cult 87, 285–295 (2006). https://doi.org/10.1007/s11240-006-9166-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-006-9166-3

Keywords

Search

Navigation