Abstract
Microtubers are used to propagate, to store, and to transport potato clones. Culturing single-node explants from potato plantletsin vitro without subtending leaves was reported to result in plantlets with lower vigor and a higher coefficient of variation. The effect on microtuber productionin vitro of leaf area and the presence or absence of leaves on potato single-node cuttings was investigated as an extension of the above study. Stock plantlets of potato cvs Atlantic, Kennebec, Russet Burbank, and Shepody were cultured under a 16-h photoperiod. Single-node cuttings were excised and grown in a high-sucrose tuberization medium in darkness. Leaf area did not affect the frequency, size, or weight of microtubers of cvs Katahdin and Russet Burbank. The absence of leaves reduced microtuber diameter for Russet Burbank; whereas Atlantic, Kennebec, and Shepody were unaffected. Mean fresh weight of microtubers was reduced when leaves were removed for all cvs except Atlantic. No effect of the removal of the leaf was observed for mean dry weights of microtubers from all cvs, although microtubers from single-node cuttings without leaves accumulated significantly more percent dry matter than those with leaves. Rapid multiplication facilities may therefore wish to consider conserving resources such as media, vessels, and growth room space by culturing explants without leaves for the production of microtubers.
Resumen
Los microtubérculos se usan para propagar, almacenar y transportar clones de papa. Se ha reportado que cultivando explantas de un solo nudo, a partir de plántulas de papain vitro sin hojas subtendidas da como resultado plántulas con poco vigor y un alto coeficiente de variación. Como una extensión del estudio anterior se investigó el efecto sobre la producción de microtubérculosin vitro, de área foliar y la presencia o ausencia de hojas en esquejes de un solo nudo. Plántulas de papa de los cultivares Atlantic, Kennebec, Russet Burbank y Shepody se cultivaron a 16 horas de fotoperiodo. Se cortaron esquejes de un solo nudo y se pusieron a crecer en un medio de tuberización con alto contenido de sucrosa y en oscuridad. El área de hoja no afectó la frecuencia, tamaño o peso de los microtubérculos en los cultivares Katahdin y Russet Burbank. La ausencia de hojas redujo el diámetro de los microtubérculos en Russet Burbank, mientras que Atlantic, Kennebec y Shepody no fueron afectados. Cuando se eliminaron las hojas no se observé reducción en el promedio de peso seco de los microtubérculos en ninguno de los cultivares, aunque los microtubérculos provenientes de esquejes de un solo nudo y sin hojas acumularon mayor porcentaje de materia seca que aquellos con hojas. Por lo tanto, para una multiplicación rápida es deseable considerar los recursos de conservación, tales como medios, envases y cámara de crecimiento para el cultivo de explantas sin hojas para producción de microtubérculos.
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Seabrook, J.E.A., Douglass, L.K. & Arnold, D.A. Effect of leaves on microtubers produced from potato single-node cuttingsIn Vitro . Am. J. Pot Res 81, 1–5 (2004). https://doi.org/10.1007/BF02853830
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DOI: https://doi.org/10.1007/BF02853830