Abstract
Aims
Cadmium (Cd) is one of the most toxic heavy metals. Cd tolerance ability differs among varieties in plants, but the underlying molecular mechanisms remain largely unknown. In this study, we identified genes that are involved in Cd stress responses and different Cd tolerances of two V. sativa varieties (Cd-tolerant variety (L3) and Cd-sensitive variety (ZM)).
Methods
Transcriptomic analysis using Illumina pair-end sequencing was carried out on root tissues of L3 and ZM grown with 5 μM and 50 μM of Cd treatments. A de novo assembled V. sativa transcriptome was generated. Differentially expressed genes (DEGs) were assigned to Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and enrichment analysis was performed. The expression of selected DEGs were confirmed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR).
Results
A total of 49,062 sequences were identified as unigenes. In the 5 μM Cd treatment, 69 and 28 differentially expressed unigenes were found as compared with the control in the L3 and ZM respectively, while in the 50 μM Cd treatment, 1036 and 335 differentially expressed unigenes were found in comparison with the control in L3 and ZM. Pathway enrichment analysis suggested that genes related to the cell wall, stress response, the glutathione pathway, metal transporters, and transcription factors are commonly up-regulated in response to Cd stress in both varieties. However, the expression of metal transporter genes and transcription factor genes showed significant differential responses to Cd stress.
Conclusions
In addition to the regulation of transcription by transcription factors, metal transporters play a vital role in controlling the different Cd tolerances of V. sativa varieties L3 and ZM.
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This work was financially supported by the National Natural Science Foundation of China (No. 31160053, 31172021), the Fundamental Research Funds for the Central Universities (KYTZ201402, KYRC201302), the Natural Science Foundation of Jiangsu Province (BK20140682) and the Natural Science Foundation of Jiangsu Higher Education (17KJB10014).
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Conceived and designed the experiments: HR, ZS, LZ. Performed the experiments: HR, XZ. Analyzed the data: HR, XZ, LZ. Manuscript draft: HR, KS, LZ. All authors have read and approved the manuscript.
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Rui, H., Zhang, X., Shinwari, K.I. et al. Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance. Plant Soil 423, 241–255 (2018). https://doi.org/10.1007/s11104-017-3501-9
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DOI: https://doi.org/10.1007/s11104-017-3501-9