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Proteomic analysis of copper stress responses in the roots of two rice (Oryza sativa L.) varieties differing in Cu tolerance

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Abstract

Background and aims

Copper (Cu) is an essential micronutrient required for growth and development of plants. However, excess Cu is toxic to plants. To understand the mechanisms involved in copper stress response, a proteomic approach was used to investigate the differences in Cu stress-induced protein expression between a Cu-tolerant variety (B1139) and a Cu-sensitive one (B1195) of rice.

Methods

Rice seedlings were exposed to 8 μM Cu for 3 days, with plants grown in the normal nutrient solution containing 0.32 μM Cu serving as the control. Proteins were extracted from the roots and separated by two-dimensional PAGE. Thirty four proteins were identified using MALDI-TOF mass spectrometry.

Results

Thirty-four protein spots were found to be differently expressed in the Cu-stressed roots in at least one variety of rice, including those involved in antioxidative defense, redox regulation, stress response, sulfur and glutathione (GSH) metabolism, carbohydrate metabolism, signal transduction, and some other proteins with various functions. Nine proteins, including putative cysteine synthase, probable serine acetyltransferase 3, L-ascorbate peroxidase 1, putative glutathione S-transferase 2, and thioredoxin-like 3-3, exhibited a greater increase in response to Cu stress in the Cu-tolerant variety B1139 compared with the Cu–sensitive variety B1195.

Conclusion

The majority of the proteins showing differential expression in response to Cu exposure are involved in the redox regulation, and sulfur and GSH metabolism, suggesting that these proteins, together with antioxidant enzymes, play an important role in the detoxification of excess Cu and maintaining cellular homeostasis.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (30471036), the Fundamental Research Funds for the Central Universities (KYT201001), the grant from the Education Department of Jiangsu (200910) and the Priority Academic Program Development of Jiangsu Higher Education Institutions

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, the People’s Republic of China

    Yufeng Song, Jin Cui, Guiping Wang & Zhenguo Shen

  2. College of Agricultural, Henan University of Science and Technology, Luoyang, 471003, the People’s Republic of China

    Hongxiao Zhang

  3. College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing, 210095, the People’s Republic of China

    Fang-Jie Zhao

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  1. Yufeng Song
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  2. Jin Cui
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  3. Hongxiao Zhang
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  4. Guiping Wang
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  5. Fang-Jie Zhao
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  6. Zhenguo Shen
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Correspondence to Zhenguo Shen.

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Song, Y., Cui, J., Zhang, H. et al. Proteomic analysis of copper stress responses in the roots of two rice (Oryza sativa L.) varieties differing in Cu tolerance. Plant Soil 366, 647–658 (2013). https://doi.org/10.1007/s11104-012-1458-2

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