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Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance

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Abstract

The ROF2 (FKBP65) is a heat stress protein which belongs to the FK506 Binding Protein (FKBP) family. It is homologous to ROF1 (FKBP62) which was recently shown to be involved in long term acquired thermotolerance by its interaction with HSP90.1 and modulation of the heat shock transcription factor HsfA2. In this study, we have demonstrated that ROF2 participates in long term acquired thermolerance, its mode of action being different from ROF1. In the absence of ROF2, the small heat shock proteins were highly expressed and the plants were resistant to heat stress, opposite to the effect observed in the absence of ROF1. It was further demonstrated that ROF2 transcription is modulated by HsfA2 which is also essential for keeping high levels of ROF2 during recovery from heat stress. ROF2 localization to the nucleus was observed several hours after heat stress exposure and its translocation to the nucleus was independent from the presence of HSP90.1 or HsfA2. ROF2 has been shown to interact with ROF1, to form heterodimers and it is suggested that via this interaction it can join the complex ROF1-HSP90.1- HsfA2. Transient expression of ROF2 together with ROF1 repressed transcription of small HSPs. A model describing the mode of action of ROF2 as a heat stress modulator which functions in negative feedback regulation of HsfA2 is proposed.

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Abbreviations

FKBP:

FK506-binding protein

PPIase:

Peptidyl prolyl cis–trans isomerase

TPR:

Tripartite tetratricopeptide repeats

HSP:

Heat shock protein

HSF:

Heat shock factor

HSE:

Heat shock element

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Acknowledgments

We thank Dr. von Koskull-Doring, Cell Biology Goethe University Frankfurt for antibodies of HsfA2 and for the HsfA2 T-DNA insertion lines Dr. Adam Institute of Plant Genetics, Hebrew University Rehovot, for antibodies of sHSP-CI. We thank Dr. Shirasu for the AtHSP90.1 T-DNA insertion lines. We thank Moran Oliva for helping with the Nuclei Isolation. We thank the Institute for Crop Improvement in Tel Aviv University for plant growing facilities. The work was supported by a grant from the Israeli National Academy of Science to A. Breiman.

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  1. Department of Plant Sciences, Tel Aviv University, 69978, Tel Aviv, Israel

    David Meiri, Keren Tazat, Reut Cohen-Peer, Odelia Farchi-Pisanty, Keren Aviezer-Hagai, Adi Avni & Adina Breiman

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  1. David Meiri
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Correspondence to Adina Breiman.

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Meiri, D., Tazat, K., Cohen-Peer, R. et al. Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Plant Mol Biol 72, 191–203 (2010). https://doi.org/10.1007/s11103-009-9561-3

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