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QTL mapping of adult-plant resistance to leaf rust in the Chinese landraces Pingyuan 50/Mingxian 169 using the wheat 55K SNP array

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Abstract

Leaf rust (LR) is a destructive foliar disease of common wheat (Triticum aestivum L.) worldwide. Growing resistant cultivars with adult plant resistance (APR) are the ideal way to control the disease. The aim of the present study was to map quantitative trait loci (QTL) for leaf rust resistance using 137 doubled-haploid (DH) lines derived from the Pingyuan 50 × Mingxian 169 cross. The same population was previously tested for stripe rust (YR) and powdery mildew (PM) response, and the data were reassessed to determine the presence of QTL with pleiotropic effects. Phenotyping for leaf rust response was conducted in six environments at Baoding in Hebei province and Zhoukou in Henan province. The DH lines were genotyped using the wheat 55K SNP array and additional SSR markers. Eight QTLs for LR resistance were identified in the present study using the inclusive composite interval mapping (ICIM). The flanking sequences of all the SNP probes were subjected to BLAST against the Chinese Spring reference sequence to determine physical positions. Six LR resistance loci were contributed by Pingyuan 50, but QLr.hebau-2DS2 and QLr.hebau-3AL were from Mingxian 169. QLr.hebau-3DS, a new and stable QTL detected in all environments, explained 4.2–20.0% of the phenotypic variation. Another new QTL, QLr.hebau-3AL detected in four environments had a PVE (phenotypic variance explained) of 5.7–10.1%. QLr.hebau-1BL, QLr.hebau-2DS2, and QLr.hebau-7DL are new loci. Two QTLs showed potential pleiotropic effects on disease response. QTL on chromosome 7DL might be pleiotropic to LR and PM, and the QTL on 5DL might confer pleiotropic resistance to LR and YR. Kompetitive allele-specific PCR (KASP) markers for QLr.hebau-3AL and QLr.hebau-3DS were successfully developed and validated. The QTL identified with their flanking markers and in the present study could be useful for fine mapping, candidate gene mining, and marker-assisted selection (MAS).

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Acknowledgments

We would like to thank Prof. R. A. McIntosh at Plant Breeding Institute, University of Sydney, for the critical review of this manuscript.

Funding

This study was supported by the National Natural Science Foundation of China (31361140367, 31571662, and 31601299).

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Authors and Affiliations

  1. College of Plant Protection, Hebei Agricultural University, 289 Lingyusi Street, Baoding, 071001, Hebei Province, China

    Peipei Zhang, Takele Weldu Gebrewahid, Zaifeng Li & Daqun Liu

  2. College of Plant Science & Technology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, 430070, Hubei Province, China

    Caixia Lan

  3. Nuclear Institute for Agriculture and Biology (NIAB), Jhang Road, Faisalabad, Pakistan

    Muhammad Azeem Asad

  4. College of Agriculture, Aksum University, 314, Shire-Indaslassie, Tigray, Ethiopia

    Takele Weldu Gebrewahid

  5. Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

    Xianchun Xia & Zhonghu He

  6. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China

    Zhonghu He

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  1. Peipei Zhang
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  2. Caixia Lan
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  3. Muhammad Azeem Asad
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  7. Zaifeng Li
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Contributions

PZ phenotyped the population in the field, prepared genotypic data, analyzed the data, and wrote the initial manuscript. CL and MA provided the phenotypic data of stripe rust and powdery mildew and SSR markers. GW modified the language. XX and ZH modified the manuscript and drafted some sections of the manuscript. ZL and DL design this experiment.

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Correspondence to Zaifeng Li or Daqun Liu.

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Zhang, P., Lan, C., Asad, M.A. et al. QTL mapping of adult-plant resistance to leaf rust in the Chinese landraces Pingyuan 50/Mingxian 169 using the wheat 55K SNP array. Mol Breeding 39, 98 (2019). https://doi.org/10.1007/s11032-019-1004-5

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