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Mapping of durable stripe rust resistance in a durum wheat cultivar Wollaroi

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Abstract

Wollaroi, an Australian durum wheat cultivar, produced a low stripe rust response and the alternative parent Bansi was highly susceptible. The Wollaroi/Bansi recombinant inbred line (RIL) population was phenotyped across three consecutive crop seasons. A genetic map of the Wollaroi/Bansi RIL population comprising 799 markers (diversity arrays technology and simple sequence repeat markers) was used to determine the genomic location of stripe rust resistance genes carried by the cultivar Wollaroi. Composite interval mapping detected three consistent quantitative trait loci (QTL) in chromosomes 2A, 3B and 5B. These QTL were named QYr.sun-2A, QYr.sun-3B and QYr.sun-5B. Another QTL, QYr.sun-1B, was detected only in the 2009 crop season. QTL in chromosomes 1B, 2A, 3B and 5B explained on average 6, 9.3, 26.7 and 8.7 %, respectively, of the variation in stripe rust response. All QTL were contributed by Wollaroi. RILs carrying these QTL singly produced intermediate stripe rust severities ranging from 46.2 to 55.7 %, whereas RILs with all four QTL produced the lowest disease severity (34.3 %). The consistently low stripe rust response of Wollaroi for 20 years demonstrated the durability of the resistance loci involved. The QTL combination detected in this study is being transferred to common wheat.

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Acknowledgments

We are grateful to Grains Research Development Corporation, Australia for funding. The award of a International Postgraduate Research Scholarship (IPRS) and International Postgraduate Award (IPA) to Baljit Singh by the University of Sydney is duly acknowledged.

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Author notes

  1. Alvina G. Kazi

    Present address: National University of Sciences and Technology (NUST), NUST Campus H-12, Islamabad, Pakistan

  2. Baljit Singh

    Present address: Punjab Agricultural University, Ludhiana, 141004, India

Authors and Affiliations

  1. University of Sydney Plant Breeding Institute-Cobbitty, Private Mail Bag 4011, Narellan, NSW, 2567, Australia

    Urmil K. Bansal, Alvina G. Kazi, Baljit Singh, Ray A. Hare & Harbans S. Bariana

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  1. Urmil K. Bansal
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Correspondence to Harbans S. Bariana.

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Bansal, U.K., Kazi, A.G., Singh, B. et al. Mapping of durable stripe rust resistance in a durum wheat cultivar Wollaroi. Mol Breeding 33, 51–59 (2014). https://doi.org/10.1007/s11032-013-9933-x

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  • DOI: https://doi.org/10.1007/s11032-013-9933-x

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