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Variation and potential value in wheat breeding of low-molecular-weight glutenin subunit genes cloned by genomic and RT-PCR in a derivative of somatic introgression between common wheat and Agropyron elongatum

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Abstract

In order to exploit the composition, origination and possible relationship with quality, 26 low-molecular-weight glutenin subunit (LMW-GS) open reading frames (ORFs) were cloned and sequenced from a derivative of an asymmetric somatic hybrid between wheat and Agropyron elongatum and parent wheat. Most of these (23) were of LMW-m type. One sequence derived from the selected derivative has a unique N-terminal peptide sequence NGRLDASL. Most of the deduced peptide sequences have eight Cys residues, but two have nine and two others ten. Reverse transcriptase polymerase chain reaction (RT-PCR) results show the LMW-GS genes that can express in the endosperm of the derivative. Lmwgh-15 from the derivative has four extra deduced Cys residues and comprises 14.6% of the total expression sequences, which may play an important role in wheat flour quality. A phylogenetic analysis, based on both deoxyribonucleic acid (DNA) and peptide sequences, indicated that most of derivative’s LMW-GS genes were inherited from the receptor wheat, but a few could be traced to the donor A. elongatum. There was evidence for both chimerism and mutation in a number of the derivative’s LMW-GS genes. We conclude that somatic hybridization is able not only to introduce alien genes but also to generate novel LMW-GS alleles.

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Acknowledgements

This work were supported by the National 863 High-Technology Research and Development Project (2006AA10Z173), Major Project and Doctor-Site Foundation of Ministry of Education in China.

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Authors and Affiliations

  1. School of Life Sciences, Shandong University, Jinan, 250100, P.R. China

    Fanguo Chen, Zhen Luo, Zhiguo Zhang, Guangmin Xia & Haoxiang Min

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  1. Fanguo Chen
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  2. Zhen Luo
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  3. Zhiguo Zhang
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  4. Guangmin Xia
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  5. Haoxiang Min
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Correspondence to Guangmin Xia.

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Fanguo Chen and Zhen Luo are equally contributed.

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Chen, F., Luo, Z., Zhang, Z. et al. Variation and potential value in wheat breeding of low-molecular-weight glutenin subunit genes cloned by genomic and RT-PCR in a derivative of somatic introgression between common wheat and Agropyron elongatum . Mol Breeding 20, 141–152 (2007). https://doi.org/10.1007/s11032-007-9081-2

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