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Enriching LMW-GS alleles and strengthening gluten properties of common wheat through wide hybridization with wild emmer

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Abstract

Two advanced lines (BAd7-209 and BAd7-213) with identical high-molecular-weight glutenin subunit composition were obtained via wide hybridization between low-gluten cultivar chuannong16 (CN16) and wild emmer D97 (D97). BAd7-209 was better than BAd7-213, and both of them were much better than CN16 in a dough quality test. We found that BAd7-209 had more abundant and higher expression levels of low-molecular-weight glutenin subunit (LMW-GS) proteins than those of BAd7-213. Twenty-nine novel LMW-GS genes at Glu-A3 locus were isolated from BAd7-209, BAd7-213 and their parents. We found that all 29 LMW-GS genes possessed the same primary structure shared by other known LMW-GSs. Twenty-seven genes encode LMW-m-type subunits, and two encode LMW-i-type subunits. BAd7-209 had a higher number of LMW-GS genes than BAd7-213, CN16, and D97. Two wild emmer genes MG574329 and MG574330 were present in the two advanced lines. Most of the LMW-m-type genes showed minor nucleotide variations between wide hybrids and their parents that could be induced through the wide hybridization process. Our results demonstrated that the wild emmer LMW-GS alleles could be feasibly transferred and integrated into common wheat background via wide hybridization and the potential value of the wild emmer LMW-GS alleles in breeding programs designed to improve wheat flour quality.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 31571668; No. 31801360), the National Key Research and Development Program of China (No. 2017YFD0100900), the Science & Technology Department of Sichuan Province (No. 2019YJ0435), and the Education Department of Sichuan Province (No. 18ZA0392).

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Author notes

  1. Lan Xiang and Lin Huang have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China

    Lan Xiang, Qiantao Jiang, Youliang Zheng, Dengcai Liu & Bihua Wu

  2. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China

    Lan Xiang, Lin Huang, Fangyi Gong, Jia Liu, Yufan Wang, Yarong Jin, Yu He, Jingshu He, Qiantao Jiang, Youliang Zheng, Dengcai Liu & Bihua Wu

  3. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, China

    Qiantao Jiang, Youliang Zheng, Dengcai Liu & Bihua Wu

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  1. Lan Xiang
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  2. Lin Huang
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  3. Fangyi Gong
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Contributions

BHW, LH, and LX designed the experiments. FYG, JL, YFW, YRJ, YH, and JSH carried out experiments. LX analyzed the data and drafted the manuscript. QTJ and YLZ provided their constructive comments and suggestions. LH, BHW, and DL revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Lin Huang or Bihua Wu.

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Xiang, L., Huang, L., Gong, F. et al. Enriching LMW-GS alleles and strengthening gluten properties of common wheat through wide hybridization with wild emmer. 3 Biotech 9, 355 (2019). https://doi.org/10.1007/s13205-019-1887-1

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